Expression profile of seven polyamine oxidase genes in rice (Oryza sativa) in response to abiotic stresses, phytohormones and polyamines

• Published in: Physiology and molecular biology of plants Vol. 27; no. 6; pp. 1353 - 1359
• Main Authors: Sagor, G. H. M., Inoue, Masataka, Kusano, Tomonobu, Berberich, Thomas
• Format: Journal Article
• Language: English
• Published: New Delhi Springer India 01.06.2021; Springer Nature B.V
• Subjects: Abscisic acid; Acetic acid; Adenine; Biological and Medical Physics; Biomedical and Life Sciences; Biophysics; Biosynthesis; Catabolism; Cell Biology; Drought; Gene expression; Genes; Gibberellic acid; Hydrogen peroxide; Indoleacetic acid; Jasmonic acid; Life Sciences; Oryza sativa; Oxidase; Oxidative stress; Phytohormones; Plant Physiology; Plant protection; Plant Sciences; Polyamine oxidase; Polyamines; Short Communication; Spermidine; Spermine; Transcription; Wounding; Polyamine; Polyamine oxidase; Hormone response; Abiotic stress; Oryza sativa
• ISSN: 0971-5894; 0974-0430
• DOI: 10.1007/s12298-021-01006-1

Polyamine levels are controlled by biosynthesis, intra- and inter-cellular flux by the respective transporters, and catabolism. The catabolism is catalyzed by two groups of enzymes. One is copper-containing amine oxidases and the other is polyamine oxidases (PAOs). In Oryza sativa , seven PAO genes exist and they are termed as OsPAO1 to OsPAO7 . However, their physiological function has not been elucidated yet. Here, we examined the expressional changes of seven OsPAO genes upon abiotic and oxidative stress, phytohormone, and exogenous polyamines application. The transcript of extracellular polyamine oxidase OsPAO2 and OsPAO6 are strongly induced upon wounding, drought, salinity, oxidative stress (H 2 O 2 ), and exogenous application of jasmonic acid, spermidine, spermine, thermospermine and negatively regulated upon indole acetic acid, isopentenyl adenine (iPT), gibberellic acid (GA), abscisic acid; OsPAO7 is to iPT, GA and all polyamines; OsPAO4 and OsPAO5 are mildly responsive to heat, cold, oxidative stress. These results suggest that polyamine oxidase encoding extracellular enzyme may play a pivotal role during exogenous stimulus to protect the plant cell.