Parathyroid suppression therapy normalizes chronic kidney disease-induced elevations in cortical bone vascular perfusion: a pilot study

• Published in: Osteoporosis international Vol. 30; no. 8; pp. 1693 - 1698
• Main Authors: Aref, M.W., Swallow, E.A., Metzger, C.E., Chen, N., Moe, S.M., Allen, M.R.
• Format: Journal Article
• Language: English
• Published: London Springer London 01.08.2019; Springer Nature B.V
• Subjects: Animal models; Animals; Blood Urea Nitrogen; Bone loss; Bone marrow; Bones; Calcification (ectopic); Calcium; Calcium - pharmacology; Calcium - therapeutic use; Cardiovascular diseases; Chronic Kidney Disease-Mineral and Bone Disorder - blood; Chronic Kidney Disease-Mineral and Bone Disorder - drug therapy; Chronic Kidney Disease-Mineral and Bone Disorder - physiopathology; Computed tomography; Cortical bone; Cortical Bone - blood supply; Dietary Supplements; Disease Models, Animal; Drug Evaluation, Preclinical - methods; Endocrinology; Femur; Kidney diseases; Male; Medicine; Medicine & Public Health; Orthopedics; Parathyroid; Parathyroid hormone; Parathyroid Hormone - antagonists & inhibitors; Parathyroid Hormone - blood; Peptides - pharmacology; Peptides - therapeutic use; Perfusion; Pilot Projects; Porosity; Rats; Regional Blood Flow - drug effects; Regional Blood Flow - physiology; Renal Insufficiency, Chronic - blood; Renal Insufficiency, Chronic - drug therapy; Renal Insufficiency, Chronic - physiopathology; Rheumatology; Short Scientific Communication; Supplements; Tibia; Urea; X-Ray Microtomography; Etelcalcitide; Bone vascular; CKD-MBD; Bone blood flow
• ISSN: 0937-941X; 1433-2965
• DOI: 10.1007/s00198-019-04974-z

Summary Interventions that alter PTH levels in an animal model of chronic kidney disease have effects on the perfusion of bone and bone marrow. Introduction Patients with chronic kidney disease (CKD) have accelerated bone loss, vascular calcification, and abnormal biochemistries, together contributing to an increased risk of cardiovascular disease and fracture-associated mortality. Despite evidence of vascular pathologies and dysfunction in CKD, our group has shown that cortical bone tissue perfusion is higher in a rat model of high-turnover CKD. The goal of the present study was to test the hypothesis that parathyroid hormone (PTH) suppressive interventions would normalize cortical bone vascular perfusion in the setting of CKD. Methods In two separate experiments, 35-week-old CKD animals and their normal littermates underwent intra-cardiac fluorescent microsphere injection to assess the effect of 10 weeks of PTH suppression (Experiment 1: calcium supplementation, Experiment 2: calcimimetic treatment) on alterations in bone tissue perfusion. Results In Experiment 1, CKD animals had serum blood urea nitrogen (BUN) and PTH levels significantly higher than NL (+ 182% and + 958%; p  < 0.05). CKD+Ca animals had BUN levels that were similar to CKD, while PTH levels were significantly lower and comparable to NL. Both femoral cortex (+ 220%, p  = 0.003) and tibial cortex (+ 336, p  = 0.005) tissue perfusion were significantly higher in CKD animals when compared to NL; perfusion was normalized to those of NL in CKD+Ca animals. MicroCT analysis of the proximal tibia cortical porosity showed a trend toward higher values in CKD (+ 401%; p  = 0.017) but not CKD+Ca (+ 111%; p  = 0.38) compared to NL. Experiment 2, using an alternative method of PTH suppression, showed similar results as those of Experiment 1. Conclusions These data demonstrate that PTH suppression-based interventions normalize cortical bone perfusion in the setting of CKD.