Prevention the formation of biofilm on orthopedic implants by melittin thin layer on chitosan/bioactive glass/vancomycin coatings

• Published in: Journal of materials science. Materials in medicine Vol. 32; no. 7; p. 75
• Main Authors: Zarghami, Vahid, Ghorbani, Mohammad, Bagheri, Kamran Pooshang, Shokrgozar, Mohammad Ali
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.07.2021; Springer Nature B.V
• Subjects: 3T3 Cells; Animals; Anti-Bacterial Agents - pharmacology; Antibacterial agents; Antibiotic resistance; Antibiotics; Antiinfectives and antibacterials; Antimicrobial Peptides - pharmacology; Bacteria; Bacterial Adhesion - drug effects; Biofilms; Bioglass; Biological activity; Biomaterials; Biomaterials Synthesis and Characterization; Biomedical Engineering and Bioengineering; Biomedical materials; Bone implants; Bone Substitutes - chemistry; Cell Proliferation; Ceramics; Ceramics - chemistry; Chemistry and Materials Science; Chitosan; Chitosan - chemistry; Coated Materials, Biocompatible - chemistry; Coatings; Composites; Drug resistance; Glass; Materials Science; Melitten - administration & dosage; Melitten - chemistry; Methicillin; Methicillin-Resistant Staphylococcus aureus - drug effects; Mice; Microscopy, Electron, Scanning; Nanoparticles; Nanoparticles - chemistry; Natural Materials; Orthopaedic implants; Orthopedics; Osteogenesis; Polymer Sciences; Powders; Prostheses and Implants; Regenerative Medicine/Tissue Engineering; Staphylococcus aureus; Staphylococcus aureus - drug effects; Staphylococcus infections; Substrates; Surface Properties; Surfaces and Interfaces; Synergistic effect; Thin Films; Titanium; Titanium - chemistry; Transplants & implants; Vancomycin; Vancomycin - administration & dosage; Vancomycin - chemistry; Vancomycin Resistance - drug effects
• ISSN: 0957-4530; 1573-4838
• DOI: 10.1007/s10856-021-06551-5

Methicillin-resistant and Vancomycin-resistant Staphylococcus aureus bacteria (MRSA and VRSA, respectively) can seriously jeopardizes bone implants. This research aimed to examine the potential synergistic effects of Melittin and vancomycin in preventing MRSA and VRSA associated bone implant infections. Chitosan/bioactive glass nanoparticles/vancomycin composites were coated on hydrothermally etched titanium substrates by casting method. The composite coatings were coated by Melittin through drop casting technique. Melittin raised the proliferation of MC3T3 cells, making it an appropriate option as osteoinductive and antibacterial substance in coatings of orthopedic implants. Composite coatings having combined vancomycin and Melittin eliminated both planktonic and adherent MRSA and VRSA bacteria, whereas coatings containing one of them failed to kill the whole VRSA bacteria. Therefore, chitosan/bioactive glass/vancomycin/Melittin coating can be used as a bone implant coating because of its anti-infective properties.