Temperate Coastal Microbial Communities Rapidly Respond to Low Concentrations of Partially Weathered Diesel

• Published in: Microbial ecology Vol. 84; no. 4; pp. 1122 - 1132
• Main Authors: Ryther, Camilla M., Ortmann, Alice C., Wohlgeschaffen, Gary, Robinson, Brian J.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.11.2022; Springer Nature B.V
• Subjects: Bacteria - genetics; Benzene; Biodegradation, Environmental; Biomedical and Life Sciences; Coastal ecosystems; Diesel; Dilution; Ecology; Environmental Microbiology; Ethylbenzene; Eukaryotes; Geoecology/Natural Processes; Hydrocarbons; Life Sciences; Low concentrations; Mesocosms; Microbial activity; Microbial Ecology; Microbiology; Microbiomes; Microbiota; Microorganisms; Nature Conservation; Nova Scotia; Petroleum; Prokaryotes; Runoff; Seawater; Seawater - microbiology; Toluene; Water Quality/Water Pollution; Wave action; Weathering; Xylene; Nova Scotia; Phytoplankton; Bacteria; Mesocosm; Diesel; Microbial community diversity; Petroleum weathering
• ISSN: 0095-3628; 1432-184X
• DOI: 10.1007/s00248-021-01939-w

Diesel is frequently encountered in coastal ecosystems due to land run-off from road surfaces. The current study investigates how partially weathered diesel at environmentally relevant concentrations, as may be seen during a run-off event, affect coastal microbial communities. A mesocosm experiment using seawater from the Bedford Basin, Nova Scotia, was followed for 72 h after the addition of partially weathered diesel. Sequencing data suggests partially weathered diesel acts quickly to alter the prokaryotic community, as both opportunistic ( Vibrio and Lentibacter ) and oil-degrading ( Colwellia , Sulfitobacter , and Pseudoalteromonas ) bacteria proliferated after 24 h in comparison to the control. In addition, total prokaryotes seemed to recover in abundance after 24 h, where eukaryotes only ceased to decrease slightly at 72 h, likely because of an inability to adapt to the oil-laden conditions, unlike the prokaryotes. Considering there were no highly volatile components (benzene, toluene, ethylbenzene, and xylene) present in the diesel when the communities were exposed, the results indicate that even a relatively small concentration of diesel run-off can cause a drastic change to the microbial community under low energy conditions. Higher energy conditions due to wave action may mitigate the response of the microbial communities by dilution and additional weathering of the diesel.