Genome sequence of the potential probiotic eukaryote Saccharomyces cerevisiae KCCM 51299

• Published in: 3 Biotech Vol. 10; no. 4; p. 185
• Main Authors: Hwang, Junsang, Bang, Ina, Kim, Donghyuk, Shin, Seung Chul, Chin, Young-Wook, Kim, Tae-Wan, Kim, Hyo Jin
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.04.2020; Springer Nature B.V
• Subjects: Agriculture; Bioinformatics; Biomaterials; Biosynthesis; Biotechnology; Cancer Research; Chemistry; Chemistry and Materials Science; DNA sequencing; Genes; Genomes; Glutathione; Metabolic engineering; Nucleotide sequence; Original; Original Article; Probiotics; Saccharomyces cerevisiae; Stem Cells; Yeast; KCCM 51299; Near-complete genome sequence; Eukaryotic probiotics; Glutathione; Saccharomyces cerevisiae KCCM 51299
• ISSN: 2190-572X; 2190-5738
• DOI: 10.1007/s13205-020-02174-9

Saccharomyces cerevisiae KCCM 51299, a potential probiotic yeast overproducing glutathione, has been isolated from among 272 yeast strains from the relatively safe Nuruk. The genome sequence of S. cerevisiae KCCM 51299 was analyzed and a near-complete genome (12 Mb) with 19 contigs was assembled after PacBio single-molecule real-time (SMRT) sequencing. The genome of S. cerevisiae KCCM 51299 was compared to the S. cerevisiae s288c reference genome. Additionally, genes involved in glutathione biosynthesis were identified, and the glutathione biosynthesis pathway was constructed in silico based on these genes. Furthermore, S. cerevisiae KCCM 51299 genes were compared with those in other yeast genomes. Finally, genome-scale in silico flux analysis was carried out, and a metabolic engineering strategy for glutathione biosynthesis was generated. These results provide useful information to further develop eukaryotic probiotics to overproduce glutathione.