Mitochondrial Oxidative Phosphorylation defect in the Heart of Subjects with Coronary Artery Disease

• Published in: Scientific reports Vol. 9; no. 1; p. 7623
• Main Authors: Ait-Aissa, Karima, Blaszak, Scott C., Beutner, Gisela, Tsaih, Shirng-Wern, Morgan, Garrett, Santos, Janine H., Flister, Michael J., Joyce, David L., Camara, Amadou K. S., Gutterman, David D., Donato, Anthony J., Porter, George A., Beyer, Andreas M.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 20.05.2019; Nature Publishing Group
• Subjects: 13/1; 38/77; 38/91; 631/443/319/333/1465; 631/443/592/75/230; 82/51; 82/80; Adenosine Triphosphate - metabolism; Cardiovascular disease; Clonal deletion; Coronary artery; Coronary Artery Disease - metabolism; Coronary vessels; DNA, Mitochondrial - metabolism; Energy Metabolism - physiology; Female; Glycolysis; Glycolysis - physiology; Heart - physiopathology; Heart diseases; Humanities and Social Sciences; Humans; Kinases; L-Lactate dehydrogenase; Lactic acid; Lysates; Male; Metabolism; Middle Aged; Mitochondria - metabolism; Mitochondrial DNA; multidisciplinary; NAD; NAD - metabolism; Oxidation-Reduction; Oxidative metabolism; Oxidative Phosphorylation; Phosphorylation; Pyruvate kinase; Pyruvic acid; Science; Science (multidisciplinary)
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-43761-y

Coronary artery disease (CAD) is a leading cause of death worldwide and frequently associated with mitochondrial dysfunction. Detailed understanding of abnormalities in mitochondrial function that occur in patients with CAD is lacking. We evaluated mitochondrial damage, energy production, and mitochondrial complex activity in human non-CAD and CAD hearts. Fresh and frozen human heart tissue was used. Cell lysate or mitochondria were isolated using standard techniques. Mitochondrial DNA ( mt DNA), NAD + and ATP levels, and mitochondrial oxidative phosphorylation capacity were evaluated. Proteins critical to the regulation of mitochondrial metabolism and function were also evaluated in tissue lysates. PCR analysis revealed an increase in mt DNA lesions and the frequency of mitochondrial common deletion, both established markers for impaired mitochondrial integrity in CAD compared to non-CAD patient samples. NAD + and ATP levels were significantly decreased in CAD subjects compared to Non-CAD (NAD + fold change: non-CAD 1.00 ± 0.17 vs. CAD 0.32 ± 0.12* and ATP fold change: non-CAD 1.00 ± 0.294 vs. CAD 0.01 ± 0.001*; N = 15, P < 0.005). We observed decreased respiration control index in CAD tissue and decreased activity of complexes I, II, and III. Expression of ETC complex subunits and respirasome formation were increased; however, elevations in the de-active form of complex I were observed in CAD. We observed a corresponding increase in glycolytic flux, indicated by a rise in pyruvate kinase and lactate dehydrogenase activity, indicating a compensatory increase in glycolysis for cellular energetics. Together, these results indicate a shift in mitochondrial metabolism from oxidative phosphorylation to glycolysis in human hearts subjects with CAD.