Calcium, zinc and vitamin E ameliorate cadmium-induced renal oxidative damage in albino Wistar rats

• Published in: Toxicology reports Vol. 3; pp. 591 - 597
• Main Authors: Adi, Pradeepkiran Jangampalli, Burra, Siva Prasad, Vataparti, Amardev Rajesh, Matcha, Bhaskar
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier 01.01.2016
• Subjects: Lipid peroxidation; Oxidative stress; PAGE analysis; Cadmium (Cd); Nephrotoxicity
• ISSN: 2214-7500; 2214-7500
• DOI: 10.1016/j.toxrep.2016.07.005

This study was aimed to examine the protective effects of supplementation with calcium + zinc (Ca + Zn) or vitamin E (Vit-E) on Cd-induced renal oxidative damage. Young albino Wistar rats (180 ± 10 g) (n = 6) control rats, Cd, Cd + Ca + Zn, and Cd + Vit-E experimental groups and the experimental period was 30 days. Rats were exposed to Cd (20 mg/kg body weight) alone treated as Cd treated group and the absence or presence of Ca + Zn (2 mg/kg each) or Vit-E (20 mg/kg body weight) supplementation treated as two separate groups. The activities of the stress marker enzymes superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and lipid peroxidase (LPx) were determined in renal mitochondrial fractions of experimental rats. We observed quantitative changes in SOD isoenzymatic patterns by non-denaturing PAGE analysis, and quantified band densities. These results showed that Cd exposure leads to decreases in SOD, CAT, GR, and GPx activities and a concomitant increase in LPx and GST activities. Ca + Zn and Vit-E administration with Cd significantly reversed Cd-induced perturbations in oxidative stress marker enzymes. However, Vit-E showed more inhibitory activity against Cd than did Ca + Zn, and it protected against Cd-induced nephrotoxicity.