Polydopamine meets porous membrane: A versatile platform for facile preparation of membrane adsorbers

• Published in: Journal of Chromatography A Vol. 1448; pp. 121 - 126
• Main Authors: Fan, Jinxin, Luo, Jianquan, Chen, Xiangrong, Wan, Yinhua
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 27.05.2016
• Subjects: Adsorption; Affinity; Affinity membrane adsorber; anion exchange; Anions; Chemical Fractionation; Chromatography; Coating; coatings; fractionation; histidine; human serum albumin; Humans; Hydrophobic and Hydrophilic Interactions; hydrophobic bonding; Hydrophobic interaction; Hydrophobicity; immunoglobulin G; Immunoglobulin G - isolation & purification; Indoles - chemistry; Ion Exchange; ion-exchange membranes; Ligands; Membrane adsorber; Membrane chromatography; Membranes; Membranes, Artificial; Polyethyleneimine; Polymers - chemistry; Porosity; Purity; Serum Albumin - isolation & purification; Sulfhydryl Compounds - isolation & purification; Surface chemistry; Hydrophobic interaction; Ion-exchange; Affinity membrane adsorber; Membrane chromatography; Membrane adsorber
• ISSN: 0021-9673; 1873-3778
• DOI: 10.1016/j.chroma.2016.04.063

•PDA-coated PES membrane was applied to fabricate various membrane adsorbers.•Anion-exchange, hydrophobic interaction and affinity membrane adsorbers were prepared.•Membrane adsorbers exhibited a high selectivity in fractionation of IgG/HSA mixture.•This work provides a promising direction for facile fabrication of membrane adsorbers. Polydopamine, as an intermediate layer coated on PES membrane, was applied to fabricate various membrane adsorbers. Anion-exchange, hydrophobic interaction and affinity membrane adsorbers prepared by this facile method exhibited a high selectivity in fractionation of IgG (immunoglobulin)/HSA (human serum albumin) mixture. The anion-exchange membrane adsorber containing polyethylenimine (PEI) improved the HSA purity from 17.7% to 96.7%; The hydrophobic interaction membrane adsorber with Dodecyl mercaptan (DDM) as ligand obtained an IgG purity of 94.6%; Histidine attached affinity membrane chromatography achieved nearly a 100% purity of IgG. The present work indicated that the polydopamine layer not only activated membrane surface to attach various adsorptive ligands under the mild condition, but also reduced non-specific adsorption. Due to the versatile conjunction function, this facile mussel-inspired coating is also promising for the preparation of diverse membrane adsorbers.