T cell–depleted cultured pediatric thymus tissue as a model for some aspects of human age-related thymus involution

• Published in: GeroScience Vol. 43; no. 3; pp. 1369 - 1382
• Main Authors: Hale, Laura P., Cheatham, Lynn, Macintyre, Andrew N., LaFleur, Bonnie, Sanders, Brittany, Troy, Jesse, Kurtzberg, Joanne, Sempowski, Gregory D.
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.06.2021; Springer Nature B.V
• Subjects: Adipose tissue; Age; Antibody microarrays; Biomedical and Life Sciences; CCL21 protein; Cell Biology; Cell culture; Chemokines; CXCL12 protein; DNA microarrays; Epithelial cells; Gene expression; Geriatrics/Gerontology; L-selectin; Life Sciences; Lymphocytes T; Microenvironments; Molecular Medicine; Original; Original Article; Thymic involution; Thymocytes; Thymus; Thymus gland; Thymus organ cultures; CXCL12; Thymus transplantation; CCL21; Thymus involution; Aging; L-selectin; CXCL16
• ISSN: 2509-2715; 2509-2723; 2509-2723
• DOI: 10.1007/s11357-020-00301-1

Human age-related thymus involution is characterized by loss of developing thymocytes and the thymic epithelial network that supports them, with replacement by adipose tissue. The mechanisms that drive these changes are difficult to study in vivo due to constant trafficking to and from the thymus. We hypothesized that the loss of thymocytes that occurs during human thymic organ cultures could model some aspects of thymus involution and begin to identify mechanisms that drive age-related changes in the thymic microenvironment. Potential mechanistically important candidate molecules were initially identified by screening conditioned media from human thymus organ cultures using antibody microarrays. These candidates were further validated using cultured tissue extracts and conditioned media. Results were compared with gene expression studies from a panel of well-characterized (non-cultured) human thymus tissues from human donors aged 5 days to 78 years. L-selectin released into conditioned media was identified as a biomarker for the content of viable thymocytes within the cultured thymus. Levels of the chemokines CCL21 and CXCL12, likely produced by surviving thymic epithelial cells, increased markedly in conditioned media as thymocytes were lost during culture. Native non-cultured thymus from adults older than 18 years also showed a strong trend toward increased CCL21 expression, in conjunction with significant decreases in thymocyte-related mRNAs compared with thymus from subjects younger than 18 years. Together, these findings demonstrate that use of postnatal human thymus organ cultures can model some aspects of human age-related thymic involution.