Excretion by subjects on a low (poly)phenol diet of phenolic gut microbiota catabolites sequestered in tissues or associated with catecholamines and surplus amino acids

• Published in: International journal of food sciences and nutrition Vol. 74; no. 4; pp. 532 - 543
• Main Authors: Pereira-Caro, Gema, Cáceres-Jimenez, Salud, Bresciani, Letizia, Mena, Pedro, Almutairi, Tahani M., Dobani, Sara, Pourshahidi, L. Kirsty, Gill, Chris I.R, Moreno Rojas, José Manuel, Clifford, Michael N., Crozier, Alan
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 19.05.2023; Taylor & Francis Ltd
• Subjects: Amino Acids; Catabolites; Catecholamines; Colon; Diet; excretion; excretion of phenolics; Fasting; Food science; Gastrointestinal Microbiome; Gut microbiota; Hippuric acid; Humans; Intestinal microflora; intestinal microorganisms; Low (poly)phenol diet; metabolites; Microbiota; Microorganisms; Nutrient deficiency; Nutrient sources; Phenol; phenolic compounds; Phenols; Phenols - metabolism; Phenylalanine; presence and absence of a Colon; Tyrosine; UHPLC-HR-MS; urine; catecholamines; phenylalanine; excretion of phenolics; Low (poly)phenol diet; UHPLC-HR-MS; presence and absence of a Colon; tyrosine
• ISSN: 0963-7486; 1465-3478; 1465-3478
• DOI: 10.1080/09637486.2023.2226369

Phenolic catabolites excreted by fasting subjects with a functioning colon and ileostomists on a low (poly)phenol diet have been investigated. Urine was collected over a 12 h fasting period after adherence to a low (poly)phenol diet for 36 h. UHPLC-HR-MS quantified 77 phenolics. Some were present in the urine of both groups in similar trace amounts and others were excreted in higher amounts by participants with a colon indicating the involvement of the microbiota. Most were present in sub- or low-µmol amounts, but hippuric acid dominated accounting on average for 60% of the total for both volunteer categories indicating significant production from sources other than non-nutrient dietary (poly)phenols. The potential origins of the phenolics associated with the low (poly)phenol diet, include endogenous catecholamines, surplus tyrosine and phenylalanine, and washout of catabolites derived from pre-study intakes of non-nutrient dietary (poly)phenols.