Identification and characterization of a novel glucomannanase from Paenibacillus polymyxa

• Published in: 3 Biotech Vol. 11; no. 3; p. 129
• Main Authors: Li, Kuikui, Jiang, Chaofeng, Tan, Haidong, Li, Junyan, Xu, Yali, Tang, Dejian, Zhao, Xiaoming, Liu, Qishun, Li, Jianguo, Yin, Heng
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.03.2021; Springer Nature B.V
• Subjects: Agriculture; Amino acids; Bioinformatics; Biomaterials; Biotechnology; Cancer Research; Chemistry; Chemistry and Materials Science; Degradation; E coli; Enzymes; Oligosaccharides; Original; Original Article; Paenibacillus polymyxa; pH effects; Stem Cells; Konjac glucomannan; Konjac glucomannan oligosaccharides; Glucomannanase; Substrate specificity; Paenibacillus polymyxa
• ISSN: 2190-572X; 2190-5738
• DOI: 10.1007/s13205-021-02676-0

Konjac glucomannan oligosaccharide has attracted much attention due to its broad biological activities. Specific glucomannan degrading enzymes are effective tools for the production of oligosaccharides from konjac glucomannan. However, there are still few reports of commercial enzymes that can specifically degrade konjac glucomannan. The gene ppgluB encoding a glucomannanase consisting of 553 amino acids (61.5 kDa) from Paenibacillus polymyxa 3–3 was cloned and heterologous expressed in Escherichia coli BL21 (DE3). The recombinant Pp GluB showed high specificity for the degradation of konjac glucomannan. Moreover, the hydrolytic products of Pp GluB degrade konjac glucomannan were a series of oligosaccharides with degrees of polymerisation of 2–12. Furthermore, the biochemical properties indicated that Pp GluB is the optimal active at 45 to 55 °C and pH 5.0–6.0, and shows highly pH stability over a very broad pH range. The present characteristics indicated that Pp GluB is a potential tool to be used to produce oligosaccharides from konjac glucomannan.