Three-dimensional super-resolution fluorescence imaging of DNA

Recent advances in fluorescence super-resolution microscopy are providing important insights into details of cellular structures. To acquire three dimensional (3D) super-resolution images of DNA, we combined binding activated localization microscopy (BALM) using fluorescent double-stranded DNA inter...

Full description

Saved in:
Bibliographic Details
Published inScientific reports Vol. 10; no. 1; p. 12504
Main Authors Yardimci, Sevim, Burnham, Daniel R., Terry, Samantha Y. A., Yardimci, Hasan
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 27.07.2020
Nature Publishing Group
Subjects
631/45/147
631/57/2265
Chromosomes
Chromosomes - metabolism
Deoxyribonucleic acid
DNA
DNA - chemistry
Fluorescence
Humanities and Social Sciences
Humans
Imaging, Three-Dimensional
Jurkat Cells
Kinetics
Localization
Metaphase
Microscopy
Microscopy, Fluorescence
Microspheres
multidisciplinary
Optical Imaging
Protein Binding
Science
Science (multidisciplinary)
Online AccessGet full text

Cover

More Information
Summary:Recent advances in fluorescence super-resolution microscopy are providing important insights into details of cellular structures. To acquire three dimensional (3D) super-resolution images of DNA, we combined binding activated localization microscopy (BALM) using fluorescent double-stranded DNA intercalators and optical astigmatism. We quantitatively establish the advantage of bis- over mono-intercalators before demonstrating the approach by visualizing single DNA molecules stretched between microspheres at various heights. Finally, the approach is applied to the more complex environment of intact and damaged metaphase chromosomes, unravelling their structural features.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-020-68892-5