Sulfakinins influence lipid composition and insulin-like peptides level in oenocytes of Zophobas atratus beetles

• Published in: Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology Vol. 192; no. 1; pp. 15 - 25
• Main Authors: Szymczak-Cendlak, M., Gołębiowski, M., Chowański, S., Pacholska-Bogalska, J., Marciniak, P., Rosiński, G., Słocińska, M.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.01.2022; Springer Nature B.V
• Subjects: Animal Physiology; Animals; Biochemistry; Biomedical and Life Sciences; Biomedicine; Carbohydrates; Cholecystokinin; Coleoptera - metabolism; Composition; Corpora allata; Corpora cardiaca; Endocrine system; Fat body; Fatty acids; Fatty Acids - metabolism; Gas chromatography; Gastrin; Hemolymph; Homeostasis; Homology; Human Physiology; Insects; Insulin; Insulins - metabolism; Larva - metabolism; Larvae; Life Sciences; Lipid composition; Lipid metabolism; Lipids; Mass spectrometry; Mass spectroscopy; Neuroendocrine system; Neuropeptides; Neuropeptides - metabolism; Oenocytes; Original Paper; Peptides; Receptors; Sulfakinin; Transcription; Ventral nerve cord; Vertebrates; Zoology; Sulfakinin receptor; Beetles; Sulfated and nonsulfated sulfakinin; Insects; Insulin-like peptides; Oenocytes; Zophobas atratus
• ISSN: 0174-1578; 1432-136X
• DOI: 10.1007/s00360-021-01398-2

Insect sulfakinins are pleiotropic neuropeptides with the homology to vertebrate gastrin/cholecystokinin peptide family. They have been identified in many insect species and affect different metabolic processes. They have a strong influence on feeding and digestion as well as on carbohydrate and lipid processing. Our study reveals that sulfakinins influence fatty acids composition in Zophobas atratus oenocytes and regulate insulin-like peptides (ILPs) level in these cells. Oenocytes are cells responsible for maintenance of the body homeostasis and have an important role in the regulation of intermediary metabolism, especially of lipids. To analyze the lipid composition in oenocytes after sulfakinins injections we used gas chromatography combined with mass spectrometry and for ILPs level determination an immunoenzymatic test was used. Because sulfakinin peptides and their receptors are the main components of sulfakinin signaling, we also analyzed the presence of sulfakinin receptor transcript (SKR2) in insect tissues. We have identified for the first time the sulfakinin receptor transcript (SKR2) in insect oenocytes and found its distribution more widespread in the peripheral tissues (gut, fat body and haemolymph) as well as in the nervous and neuro-endocrine systems (brain, ventral nerve cord, corpora cardiaca/corpora allata CC/CA) of Z. atratus larvae. The presence of sulfakinin receptor transcript (SKR2) in oenocytes suggests that observed effects on oenocytes lipid and ILPs content may result from direction action of these peptides on oenocytes.