Nanohybrid electrochemical enzyme sensor for xanthine determination in fish samples

• Published in: 3 Biotech Vol. 11; no. 5; p. 212
• Main Authors: Sharma, Nirmal Kant, Monika, Kaushal, Ankur, Thakur, Shikha, Thakur, Neerja, Sheetal, Kumar, Dinesh, Bhalla, Tek Chand
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.05.2021; Springer Nature B.V
• Subjects: Agriculture; Bioinformatics; Biomaterials; Biosensors; Biotechnology; Cancer Research; Carbodiimide; Chemistry; Chemistry and Materials Science; Coated electrodes; Electrical measurement; Electrodes; Fish; Gold; Immobilization; Meat; Multi wall carbon nanotubes; Nanoparticles; Nanotubes; Original; Original Article; Stem Cells; Xanthine oxidase; Xanthine oxidase (XO); RL-2d; Amperometric biosensor; Bacilluspumilus RL-2d
• ISSN: 2190-572X; 2190-5738
• DOI: 10.1007/s13205-021-02735-6

An amperometric biosensor for xanthine was designed, based on covalent immobilization of xanthine oxidase (XO) of Bacillus pumilus RL-2d onto a screen-printed multi-walled carbon nanotubes gold nanoparticle-based electrodes (Nano-Au/c-MWCNT). The carboxyl groups at the electrode surface were activated by the use of 1-Ethyl-3-(3-dimethylaminopropyl carbodiimide) (EDC) and N-hydroxysuccinimide (NHS). The working electrode was then coated with 6 μL of xanthine oxidase (0.273 U/mg protein). The cyclic voltammetry (CV) study was done for the characterization of the sensor using [K 3 Fe(CN) 6 ] as an artificial electron donor. The sensitivity (S) and the limit of detection (LOD) of the biosensor were 2388.88 µA/cm 2 /nM (2.388 µA/cm 2 /µM) and 1.14 nM, respectively. The developed biosensor was used for determination of fish meat freshness.