Possible requirement of intercellular adhesion molecule-1 for invasion of gingival epithelial cells by Treponema medium

• Published in: Canadian journal of microbiology Vol. 53; no. 11; pp. 1232 - 1238
• Main Authors: Tamai, Riyoko, Asai, Yasuyuki, Kawabata, Atsushi, Akisaka, Toshitaka, Ogawa, Tomohiko
• Format: Journal Article
• Language: English
• Published: Ottawa, ON National Research Council of Canada 01.11.2007; NRC Research Press
• Subjects: Bacteriology; Biological and medical sciences; Cell adhesion molecules; Cell Line; cellules épithéliales gingivales; Culture Media; Epithelial cells; Epithelial Cells - chemistry; Epithelial Cells - metabolism; Epithelial Cells - microbiology; Fundamental and applied biological sciences. Psychology; Gingiva - microbiology; Gingiva - pathology; gingival epithelial cells; Humans; intercellular adhesion molecule-1; Intercellular Adhesion Molecule-1 - physiology; maladie parodontale; Microbiological research; Microbiology; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Periodontal disease; Periodontitis - microbiology; Physiological aspects; Properties; RNA Interference; Treponema; Treponema - pathogenicity; Treponema - ultrastructure; Treponema medium; Treponemal Infections - physiopathology; Canada; Periodontal disease; Spirochaetales; Intercellular adhesion molecule 1; Stomatology; Bacteria; Epithelial cell; Treponemataceae; Gingiva; Immunofluorescence; Adhesion; Invasion; Treponema
• ISSN: 0008-4166; 1480-3275
• DOI: 10.1139/W07-094

Oral treponemes are members of the spirochete family of bacteria associated with periodontal diseases. In the present study, we demonstrate that intercellular adhesion molecule-1 (ICAM-1) on human gingival epithelial cells (HGEC) contributed to the invasion of Treponema medium , a medium-sized oral Treponema, into those cells. The quantity of T. medium in HGEC was found to peak at 2 h after inoculation and then decreased gradually. Immunofluorescence microscopy findings showed that the bacteria were colocalized with ICAM-1 on HGEC. Furthermore, knockdown of ICAM-1 in HGEC resulted in inhibition of T. medium invasion by RNA interference, whereas that of Toll-like receptor 2 did not. These results suggest that ICAM-1 may be required for the invasion of T. medium into HGEC, and they indicate that the molecule plays a principal role in the primary stages of the development and progression of chronic periodontitis.