Developmental regulation and phytochrome-mediated induction of mRNAs encoding a proline-rich protein, glycine-rich proteins, and hydroxyproline-rich glycoproteins in Phaseolus vulgaris L

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 90; no. 3; pp. 828 - 832
• Main Authors: Sheng, J. (University of Texas, Austin, TX), Jeong, J, Mehdy, M.C
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 01.02.1993; National Acad Sciences; National Academy of Sciences
• Subjects: Agronomy. Soil science and plant productions; ARN MENSAJERO; ARN MESSAGER; Beans; Biological and medical sciences; Botany; Cell walls; CODE GENETIQUE; CODIGO GENETICO; Cotyledons; Epicotyls; EXPRESION GENICA; EXPRESSION DES GENES; Fundamental and applied biological sciences. Psychology; Gene expression regulation; GLICINA; GLICOPROTEINAS; GLYCINE (ACIDE AMINE); GLYCOPROTEINE; HIDROXIPROLINA; HYDROXYPROLINE; Hypocotyls; LUMIERE; LUZ; Messenger RNA; MORFOGENESIS; MORPHOGENESE; PHASEOLUS VULGARIS; Physical agents; PIGMENT; PIGMENTOS; Plant physiology and development; Plants; PLANTULAS; PLANTULE; PROLINA; PROLINE; PROTEINAS VEGETALES; PROTEINE VEGETALE; Proteins; RACINE; RAICES; Ribonucleic acid; RNA; Seedlings; Soybeans; Vegetative apparatus, growth and morphogenesis. Senescence; Light effect; Gene expression; Cell wall; Morphogenesis; Proteins; Regulation(control); Messenger RNA; Leguminosae; Phaseolus vulgaris; Dicotyledones; Angiospermae; Phytochrome; Development; Spermatophyta; Localization; Chromoprotein
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.90.3.828

We have studied developmental and light regulation of mRNAs encoding a putative cell wall proline-rich protein (PvPRP1), cell wall glycine-rich proteins (GRPs), and cell wall hydroxyproline-rich glycoproteins (HRGPs) in bean (Phaseolus vulgaris). Light increases the levels of these mRNAs 2- to 150-fold in highly spatially regulated patterns during seedling development. These mRNA changes include differential regulation of transcripts derived from the GRP and HRGP multigene families. In 6-day-old light-grown seedlings, the PvPRP1 and GRP1.0 mRNAs were most abundant in the apical region of hypocotyls, epicotyls, and roots. In contrast, several HRGP transcripts were most abundant in the mature region of hypocotyls and roots in light-grown seedlings. When etiolated 6-day-old seedlings were illuminated with white light for 8 hr, maximal accumulation of PvPRP1 and GRP1.0 mRNAs occurred in the apical hook, whereas HRGP and GRP1.8 mRNAs accumulated in the mature region of hypocotyls. Etiolated seedlings subjected to a pulse of red light accumulated PvPRP1, GRP, and HRGP mRNAs in the hypocotyls. Far-red light inhibited red light induction of these mRNAs, indicating a phytochrome-mediated process. The possible roles of PRPs, GRPs, and HRGPs in cell differentiation and photomorphogenesis are discussed.