HPLC isolation of the anti-plasmodially active bisbenzylisoquinone alkaloids present in roots of Cissampelos mucronata

• Published in: Phytochemical analysis Vol. 14; no. 1; pp. 13 - 22
• Main Authors: Tshibangu, J.N, Wright, A.D, Konig, G.M
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 2003
• Subjects: alkaloids; Alkaloids - isolation & purification; Alkaloids - pharmacology; Animals; anti-malarial; anti-plasmodial; antimalarials; Antimalarials - isolation & purification; Antimalarials - pharmacology; Benzylisoquinolines; bisbenzylisoquinoline alkaloids; bisbenzylisoquinone alkaloids; cell lines; Cells, Cultured; chemical analysis; chemical structure; Chromatography, High Pressure Liquid; Cissampelos; Cissampelos - chemistry; Cissampelos mucronata; cytotoxicity; gas chromatography; high performance liquid chromatography; HPLC; humans; isolation; Isoquinolines - isolation & purification; Isoquinolines - pharmacology; Magnoliaceae; mass spectrometry; medicinal plants; Molecular Structure; natural product isolation; phytochemicals; plant extracts; Plant Roots - chemistry; Plasmodium falciparum; Plasmodium falciparum - drug effects; qualitative analysis; quantitative analysis; roots; Schisandra chinensis; spectral analysis; volatile compounds; China; Republic of the Congo
• ISSN: 0958-0344; 1099-1565
• DOI: 10.1002/pca.673

The methanolic extract of dried, powdered Cissampelos mucronata roots possesses significant in vitro activity against Plasmodium falciparum. In order to enable further pharmacological testing, the substances responsible for the observed activity were purified, mainly by HPLC, using various stationary and mobile phases. The active principles were determined to be a series of bisbenzylisoquinoline alkaloids, a group of natural products for which one of the first routine preparative HPLC separation methods is described. Copyright © 2003 John Wiley & Sons, Ltd.