Efficient tissue-specific expression of bovine alpha-lactalbumin in transgenic mice

Transgenic mice carrying a 3.2‐kb genomic fragment comprising the bovine α‐lactalbumin gene transcription unit with 750 bp and 336 bp of 5′ and 3′ flanking regions, respectively, were generated. Tissue‐specific expression of the transgene was observed in five out of the six lines analyzed at levels...

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Bibliographic Details
Published inEuropean Journal of Biochemistry Vol. 186; no. 1/2; pp. 43 - 48
Main Authors Vilotte, J.L. (Institut National de la Recherche Agronomique, Jouy-en-Josas, France), Soulier, S, Stinnakre, M.G, Massoud, M, Mercier, J.C
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 01.12.1989
Wiley
Subjects
ALBUMINAS
ALBUMINE
ALBUMINS
Animals
Biochemistry, Molecular Biology
Blotting, Western
BOVIN
CATTLE
Galactosyltransferases - metabolism
GANADO BOVINO
GENOMAS
GENOME
GENOMES
GLANDE MAMMAIRE
GLANDULAS MAMARIAS
Lactalbumin - analysis
Lactalbumin - genetics
Lactalbumin - metabolism
LACTOSA
LACTOSE
Life Sciences
MAMMARY GLANDS
MICE
Mice, Transgenic - genetics
Mice, Transgenic - metabolism
Milk - analysis
MILK PROTEINS
PROTEINAS DE LA LECHE
PROTEINE DU LAIT
RATON
RNA - analysis
SOURIS
SYNTHESIS
Transcription, Genetic
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Summary:Transgenic mice carrying a 3.2‐kb genomic fragment comprising the bovine α‐lactalbumin gene transcription unit with 750 bp and 336 bp of 5′ and 3′ flanking regions, respectively, were generated. Tissue‐specific expression of the transgene was observed in five out of the six lines analyzed at levels close to the endogenous gene in two of them.
Bibliography:L50
9019456
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0014-2956
1432-1033
1432-1327
DOI:10.1111/j.1432-1033.1989.tb15175.x