Solvent extraction of bacteriocins from liquid cultures

• Published in: Letters in applied microbiology Vol. 31; no. 3; pp. 193 - 197
• Main Authors: Burianek, L.L., Yousef, A.E.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.09.2000; Blackwell Science
• Subjects: Ammonium Sulfate; bacillicin; Bacillus subtilis; Bacteriocins - chemistry; Bacteriocins - isolation & purification; Bacteriocins - pharmacology; Bacteriological methods and techniques used in bacteriology; Bacteriology; Biological and medical sciences; Chemical Precipitation; Chloroform; Culture Media - chemistry; Fundamental and applied biological sciences. Psychology; Gram-Positive Bacteria; lacidin; Lactobacillus acidophilus; Lactobacillus acidophilus - growth & development; Microbiology; Solvents; subtilin; Lactic acid bacteria; Bacteriocin; Bacteria; Lactobacillaceae; Method; Solvent extraction; Lactobacillus acidophilus; Antimicrobial agent
• ISSN: 0266-8254; 1472-765X; 1365-2673
• DOI: 10.1046/j.1365-2672.2000.00802.x

A solvent extraction method was developed to concentrate lacidin from the culture of Lactobacillus acidophilus OSU133. The new method concentrates the bacteriocin at the interface between chloroform and the aqueous culture of the producing bacterium. Compared with other extraction procedures, the new method effectively recovers higher bacteriocin yield and results in relatively clean preparations. Recovery of lacidin by the chloroform extraction procedure, compared with ammonium sulphate precipitation and cell acidification methods, was >10‐fold and about 100‐fold greater, respectively. The new extraction procedure saves time and is easy to perform. This method is also effective in recovering subtilin, bacillicin, pediocin and nisin from cultures of Bacillus subtilis ATCC 6633, B. subtilis OSY1115/C, Pediococcus acidilactici PO2 and Lactococcus lactis ATCC 11454, respectively.