Granulocyte colony‐stimulating factor (G‐CSF) receptor gene expression of ovarian carcinoma does not correlate with G‐CSF caused cell proliferation

BACKGROUND Recombinant human granulocyte colony‐stimulating factor (rhG‐CSF) is a growth factor commonly used to avoid leukopenia after chemotherapy. Endogenous G‐CSF is produced by macrophages and granulocytes that infiltrate tumors. It has been reported that rhG‐CSF stimulates the proliferation of...

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Published inCancer Vol. 91; no. 7; pp. 1372 - 1383
Main Authors Brandstetter, Thomas, Ninci, Elena, Bettendorf, Herta, Perewusnyk, Gloria, Stolte, Jens, Herchenbach, Dieter, Sellin, Dieter, Wagner, Edgar, Köchli, Ossi R., Bauknecht, Thomas
Format Journal Article
LanguageEnglish
Published New York John Wiley & Sons, Inc 01.04.2001
Wiley-Liss
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Summary:BACKGROUND Recombinant human granulocyte colony‐stimulating factor (rhG‐CSF) is a growth factor commonly used to avoid leukopenia after chemotherapy. Endogenous G‐CSF is produced by macrophages and granulocytes that infiltrate tumors. It has been reported that rhG‐CSF stimulates the proliferation of several cell lines as well as bladder carcinoma cells. Conversely, in some hematopoietic cell lines such as U‐937, WEHI‐3B, and K‐562 no effect or in some cases a differentiation pattern was found. Moreover, the role of rhG‐CSF on the proliferation of solid tumors is not well understood. METHODS In this study, 10 ovarian carcinoma biopsies were characterized for the presence of G‐CSF and G‐CSF receptor by reverse transcription–polymerase chain reaction (RT‐PCR) and immunohistochemical analysis. Proliferation was analyzed by ATP viability assays. RESULTS Performing RT‐PCR, these biopsies and four ovarian carcinoma cell lines were analyzed for endogenous G‐CSF production, which was found in some biopsies and in all cell lines. Despite the presence of the G‐CSF receptor in all biopsies and cell lines, no proliferation was found after rhG‐CSF incubation of the cell lines or the tumor samples for 3 and for 6 days, respectively. CONCLUSIONS Summarizing the authors' in vitro studies, rhG‐CSF does not affect the proliferation of ovarian carcinoma cells in vitro. Cancer 2001;91:1372–83. © 2001 American Cancer Society. Granulocyte colony‐stimulating factor shows less effect on the proliferation of ovarian carcinoma cells by adenosine triphosphate viability assay.
Bibliography:Fax: 497hyphen;761‐5038‐319
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ISSN:0008-543X
1097-0142
DOI:10.1002/1097-0142(20010401)91:7<1372::AID-CNCR1141>3.0.CO;2-E