Granulocyte colony‐stimulating factor (G‐CSF) receptor gene expression of ovarian carcinoma does not correlate with G‐CSF caused cell proliferation

• Published in: Cancer Vol. 91; no. 7; pp. 1372 - 1383
• Main Authors: Brandstetter, Thomas, Ninci, Elena, Bettendorf, Herta, Perewusnyk, Gloria, Stolte, Jens, Herchenbach, Dieter, Sellin, Dieter, Wagner, Edgar, Köchli, Ossi R., Bauknecht, Thomas
• Format: Journal Article
• Language: English
• Published: New York John Wiley & Sons, Inc 01.04.2001; Wiley-Liss
• Subjects: Adenosine Triphosphate - analysis; ATP viability assay; Biological and medical sciences; Biopsy; Blotting, Southern; Carcinoma - chemistry; Carcinoma - genetics; Carcinoma - pathology; Cell Division; Colony-Stimulating Factors - pharmacology; Female; Female genital diseases; Gene Expression; Granulocyte Colony-Stimulating Factor - analysis; Granulocyte Colony-Stimulating Factor - physiology; granulocyte colony‐stimulating factor receptor; Gynecology. Andrology. Obstetrics; Humans; Immunohistochemistry; Medical sciences; ovarian carcinoma; Ovarian Neoplasms - chemistry; Ovarian Neoplasms - genetics; Ovarian Neoplasms - pathology; proliferation; Receptors, Granulocyte Colony-Stimulating Factor - analysis; Receptors, Granulocyte Colony-Stimulating Factor - genetics; Recombinant Proteins; Reverse Transcriptase Polymerase Chain Reaction; Tumor Cells, Cultured; Tumors; Human; Cell proliferation; Carcinoma; Cytokine; Malignant tumor; Gene expression; In vitro; Female genital diseases; Ovarian diseases; Ovary; Granulocyte colony stimulating factor; Growth factor receptor; Polypeptide; Established cell line; Genetics; Biological receptor
• ISSN: 0008-543X; 1097-0142
• DOI: 10.1002/1097-0142(20010401)91:7<1372::AID-CNCR1141>3.0.CO;2-E

BACKGROUND Recombinant human granulocyte colony‐stimulating factor (rhG‐CSF) is a growth factor commonly used to avoid leukopenia after chemotherapy. Endogenous G‐CSF is produced by macrophages and granulocytes that infiltrate tumors. It has been reported that rhG‐CSF stimulates the proliferation of several cell lines as well as bladder carcinoma cells. Conversely, in some hematopoietic cell lines such as U‐937, WEHI‐3B, and K‐562 no effect or in some cases a differentiation pattern was found. Moreover, the role of rhG‐CSF on the proliferation of solid tumors is not well understood. METHODS In this study, 10 ovarian carcinoma biopsies were characterized for the presence of G‐CSF and G‐CSF receptor by reverse transcription–polymerase chain reaction (RT‐PCR) and immunohistochemical analysis. Proliferation was analyzed by ATP viability assays. RESULTS Performing RT‐PCR, these biopsies and four ovarian carcinoma cell lines were analyzed for endogenous G‐CSF production, which was found in some biopsies and in all cell lines. Despite the presence of the G‐CSF receptor in all biopsies and cell lines, no proliferation was found after rhG‐CSF incubation of the cell lines or the tumor samples for 3 and for 6 days, respectively. CONCLUSIONS Summarizing the authors' in vitro studies, rhG‐CSF does not affect the proliferation of ovarian carcinoma cells in vitro. Cancer 2001;91:1372–83. © 2001 American Cancer Society. Granulocyte colony‐stimulating factor shows less effect on the proliferation of ovarian carcinoma cells by adenosine triphosphate viability assay.