The combined effect of photoperiod, light intensity and GA3 on adventitious shoot regeneration from cotyledons of spinach (Spinacia oleracea L.)

• Published in: Plant Biotechnology Vol. 23; no. 4; pp. 431 - 435
• Main Authors: Geekiyanage, Sudarshanee, Takase, Tomoyuki, Watanabe, Shin, Fukai, Seiichi, Kiyosue, Tomohiro
• Format: Journal Article
• Language: English
• Published: Tokyo Japanese Society for Plant Cell and Molecular Biology 2006; Japan Science and Technology Agency
• Subjects: GA3; Light intensity; photoperiod; regeneration; Seedlings; spinach; Variance analysis
• ISSN: 1342-4580; 1347-6114
• DOI: 10.5511/plantbiotechnology.23.431

The effects of photoperiod, light intensity and gibberellic acid (GA3) on adventitious shoot regeneration from spinach cotyledons were tested. The Analysis of Variance F test showed that the effects of photoperiod and GA3 on shoot regeneration were significant at high light intensity of 90–100 μmol m−2 s−1. The combined effect of optimum shoot regeneration and highest shoot multiplication was observed in explants of short day (SD)-grown seedlings cultured under the SD condition and at high light intensity, with 0.5 mg l−1 GA3 in Murashige and Skoog medium supplemented with 1 mg l−1 6-benzyladenine and 0.4 mg l−1 α-naphthaleneacetic acid. Scanning electron microscopy observation revealed multiple shoots regenerating directly from the basal end of cotyledon preceding callus. The regenerated shoots developed into normal plantlets and grew well upon transfer to pots. Some regenerated shoots flowered in vitro under the non-inductive SD condition.