In vivo fluorescence lifetime imaging of macrophage intracellular metabolism during wound responses in zebrafish

• Published in: eLife Vol. 11
• Main Authors: Miskolci, Veronika, Tweed, Kelsey E, Lasarev, Michael R, Britt, Emily C, Walsh, Alex J, Zimmerman, Landon J, McDougal, Courtney E, Cronan, Mark R, Fan, Jing, Sauer, John-Demian, Skala, Melissa C, Huttenlocher, Anna
• Format: Journal Article
• Language: English
• Published: England eLife Sciences Publications Ltd 24.02.2022; eLife Sciences Publications, Ltd
• Subjects: Adenine; Animals; Cell Biology; Coenzymes; Female; Flavin; Flavin-adenine dinucleotide; Flavin-Adenine Dinucleotide - metabolism; FLIM; Fluorescence; Glucose; Glycolysis; Immunology and Inflammation; immunometabolism; Intracellular; Kinetics; Lifetime; Macrophages; Macrophages - metabolism; Metabolism; Metformin; Mice; Mice, Inbred C57BL; Microenvironments; Microscopy, Fluorescence, Multiphoton - methods; NAD(P)H; NADP - metabolism; NADPH; optical redox ratio; Oxidation-Reduction; Phosphorylation; Proteins; Redox properties; Stat6 protein; Thermal injury; Tumor Necrosis Factor-alpha - metabolism; Tumor necrosis factor-α; Wound healing; Wounds; Wounds and Injuries - metabolism; Zebrafish - metabolism; mouse; macrophages; wound healing; cell biology; optical redox ratio; immunometabolism; inflammation; FLIM; NAD(P)H; zebrafish; immunology

The function of macrophages in vitro is linked to their metabolic rewiring. However, macrophage metabolism remains poorly characterized in situ. Here, we used two-photon intensity and lifetime imaging of autofluorescent metabolic coenzymes, nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) and...