Crystallization and preliminary crystallographic analysis of the bacterial capsule assembly-regulating tyrosine phosphatases Wzb of Escherichia coli and Cps4B of Streptococcus pneumoniae

• Published in: Acta crystallographica. Section F, Structural biology and crystallization communications Vol. 65; no. 8; pp. 770 - 772
• Main Authors: Huang, Hexian, Hagelueken, Gregor, Whitfield, Chris, Naismith, James H.
• Format: Journal Article
• Language: English
• Published: 5 Abbey Square, Chester, Cheshire CH1 2HU, England International Union of Crystallography 01.08.2009
• Subjects: Base Sequence; Cps4B; Crystallization; Crystallization Communications; Crystallography, X-Ray; DNA Primers; Escherichia coli; Escherichia coli - enzymology; Escherichia coli Proteins - chemistry; Escherichia coli Proteins - genetics; kinases; phosphatases; Phosphoprotein Phosphatases - chemistry; Phosphoprotein Phosphatases - genetics; Polymerase Chain Reaction; Protein Tyrosine Phosphatases - chemistry; Protein Tyrosine Phosphatases - genetics; Recombinant Proteins - chemistry; Streptococcus; Streptococcus pneumoniae; Streptococcus pneumoniae - enzymology; Wzb
• ISSN: 1744-3091; 1744-3091
• DOI: 10.1107/S1744309109023914

Bacterial tyrosine kinases and their cognate phosphatases are key players in the regulation of capsule assembly and thus are important virulence determinants of these bacteria. Examples of the kinase/phosphatase pairing are found in Gram‐negative bacteria such as Escherichia coli (Wzc and Wzb) and in Gram‐positive bacteria such as Streptococcus pneumoniae (CpsCD and CpsB). Although Wzb and Cps4B are both predicted to dephosphorylate the C‐terminal tyrosine cluster of their cognate tyrosine kinase, they appear on the basis of protein sequence to belong to quite different enzyme classes. Recombinant purified proteins Cps4B of S. pneumoniae TIGR4 and Wzb of E. coli K‐30 have been crystallized. Wzb crystals belonged to space‐group family P3x21 and diffracted to 2.7 Å resolution. Crystal form I of Cps4B belonged to space‐group family P4x212 and diffracted to 2.8 Å resolution; crystal form II belonged to space group P212121 and diffracted to 1.9 Å resolution.