Identifying mRNAs Bound by Human RBMY Protein in the Testis

Rbmy gene encodes a germ-cell specific nuclear RNA-binding protein and is involved in spermatogenesis. To further investigate the specific events of spermatogenesis in which Rbmy plays a role, the target mRNAs of human RBMY protein were isolated and identified. Through the isolating specific nucleic...

Full description

Saved in:
Bibliographic Details
Published inJournal of Reproduction and Development Vol. 57; no. 1; pp. 107 - 112
Main Authors ZENG, Mei, LIANG, Suhua, ZHAO, Shaozhi, LIU, Yanyan, SUN, Huaqin, ZHANG, Sizhong, MA, Yongxin
Format Journal Article
LanguageEnglish
Published Japan THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT 01.02.2011
Subjects
Alternative Splicing
Electrophoretic Mobility Shift Assay
Gene Expression Profiling
Humans
Male
Nek10
NIMA-Related Kinases
Nuclear Proteins - metabolism
Protein Binding
Protein-Serine-Threonine Kinases - genetics
Protein-Serine-Threonine Kinases - metabolism
Rbmy gene
Recombinant Fusion Proteins - metabolism
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - isolation & purification
RNA, Messenger - metabolism
RNA-binding protein
RNA-Binding Proteins - metabolism
SNAAP technique
Spermatogenesis
Testis - metabolism
Online AccessGet full text

Cover

More Information
Summary:Rbmy gene encodes a germ-cell specific nuclear RNA-binding protein and is involved in spermatogenesis. To further investigate the specific events of spermatogenesis in which Rbmy plays a role, the target mRNAs of human RBMY protein were isolated and identified. Through the isolating specific nucleic acids associated with proteins (SNAAP) technique, we isolated twenty potential target genes of human RBMY protein from the human testis in the present study. Some of these target genes play important roles during spermatogenesis and have alternative transcripts in the testis. In this study, we focused on the human- related (never in mitosis gene a) kinase 10 (Nek10) gene, which belongs to the Nek protein kinase subfamily. Nek10 has two transcripts, and the results of RT-PCR and Electrophoretic Mobility Shift Assays (EMSA) show that hRBMY protein can only bind to transcript variant 2 of Nek10 and that hRbmy may take part in alternative splicing of Nek10. Isolation and identification of target genes of hRBMY will be helpful to further investigate the biological function of RBMY in spermatogenesis.
ISSN:0916-8818
1348-4400
DOI:10.1262/jrd.10-092N