Differential dopaminergic regulation of inwardly rectifying potassium channel mediated subthreshold dynamics in striatal medium spiny neurons

• Published in: Neuropharmacology Vol. 107; pp. 396 - 410
• Main Authors: Zhao, Bo, Zhu, Junling, Dai, Dongqing, Xing, Junling, He, Jiahou, Fu, Zhanyan, Zhang, Lei, Li, Zhuyi, Wang, Wenting
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.08.2016
• Subjects: Animals; Corpus Striatum - metabolism; Dendritic integration; Dopamine D2 Receptor Antagonists - pharmacology; Dopamine receptor; Dopaminergic Neurons - metabolism; Dorsal striatum; Female; Male; Medium-sized spiny neurons; Membrane resonance; Mice; Mice, Transgenic; Organ Culture Techniques; Patch clamp; Potassium Channels, Inwardly Rectifying - physiology; Receptors, Dopamine D1 - antagonists & inhibitors; Receptors, Dopamine D1 - metabolism; Receptors, Dopamine D2 - metabolism; Membrane resonance; Patch clamp; Medium-sized spiny neurons; Dendritic integration; Dopamine receptor; Dorsal striatum
• ISSN: 0028-3908; 1873-7064
• DOI: 10.1016/j.neuropharm.2016.03.037

The dorsal striatum plays a key role in motor control and cognitive processes. Proper functioning of the striatum relies on the fine dynamic balance between the direct pathway projection medium spiny neurons (MSNs) that express D1 dopamine receptor (D1 MSNs) and indirect pathway projection MSNs that express D2 dopamine receptor (D2 MSNs). The inwardly rectifying K+ channels (Kir), which express on both D1 and D2 MSNs, participate in the subthreshold dynamics including the membrane resonance and dendritic integration. However, it remains unclear whether dopamine differentially regulates Kir mediated subthreshold dynamics in two subtypes MSNs. Using transgenic mice that express either tdTomato in D1 MSNs or eGFP in D2 MSNs, we explored the Kir mediated subthreshold dynamics in D1 or D2 MSNs with whole cell patch clamp recording in acute brain slices. We found that D1 receptor agonist increased the Kir current while D2 receptor activation decreased the Kir conductance. The dopamine regulation of the Kir enhanced the resonant frequency and reduced the resonant impedance of D1 MSNs. The converse is ture for D2 MSNs. It also caused an opposing effect on dendritic integration between D1 and D2 MSNs, which can promote stability of the two pathways. The D1 receptor activation modulated Kir through cAMP-PKA signaling, whereas the D2 receptor modulated Kir through PLC-PKC signaling. Our findings demonstrated the differential dopaminergic regulation role of Kir, which mediates distinct subthreshold dynamics, and thus, contributes to the role of dopamine in fine tuning the balance of the striatal direct and indirect pathway activities. •Dopamine (DA) differentially regulated Kir in D1 and D2 MSNs in dorsal striatum.•DA differentially regulated Kir mediated β-membrane resonance in D1 and D2 MSNs.•DA differentially regulated Kir mediated dendritic integration in D1 and D2 MSNs.•DA enhanced Kir conductance through cAMP-PKA pathway in D1 MSNs.•DA inhibited Kir conductance through PLC-PKC pathway in D2 MSNs.