Role of antibodies to human papillomavirus 16 in prostate cancer: A seroscreening by peptide microarray

• Published in: Tumor biology Vol. 39; no. 6; p. 101042831769837
• Main Authors: Zhao, Xiaojun, Zhou, Zheng, Chen, Ye, Chen, Wen, Ma, Hongwei, Pu, Jinxian
• Format: Journal Article
• Language: English
• Published: London, England SAGE Publications 01.06.2017; Sage Publications Ltd; SAGE Publishing
• Subjects: Aged; Antibodies; Antibodies, Viral - isolation & purification; Antigens; Authorship; Carcinogenesis; Deoxyribonucleic acid; DNA; Human papillomavirus; Human papillomavirus 16 - isolation & purification; Human papillomavirus 16 - pathogenicity; Humans; Immunoglobulins; Infections; Male; Papillomaviridae; Papillomavirus Infections - pathology; Papillomavirus Infections - virology; Peptides; Prostate - pathology; Prostate - virology; Prostate cancer; Prostatic Neoplasms - pathology; Prostatic Neoplasms - virology; Protein Array Analysis; Proteins; Risk Factors; Serogroup; Beijing China; Asia; China; seroscreening; peptide microarray; human papillomavirus 16; Prostate cancer
• ISSN: 1010-4283; 1423-0380; 1423-0380
• DOI: 10.1177/1010428317698371

Evidence is accumulating in estimating the potential role of human papillomavirus infection in prostate carcinogenesis. However, the results remain inconclusive. We measured the serostatus of antibodies to one of the high-risk human papillomaviruses, human papillomavirus 16, with a newly developed peptide microarray. Serum samples were collected from 75 untreated prostate cancer patients, along with 80 control subjects. We identified 12 peptides with significant differences in prostate cancer samples from all 241 peptides derived from human papillomavirus 16. Our results showed human papillomavirus 16 infection in 64.0% of prostate cancer serum samples, which is significantly different compared with the controls (p < 0.01) because only 17.5% of the control serum was considered seropositive. The area under the receiver operator characteristic curve was 0.793 (95% confidence interval 0.721–0.864), indicating that the new microarray technique may have diagnostic value. The results showed an association between serological evidence for human papillomavirus 16 infection and risk of prostate cancer. The different serostatus of antibodies in the two subgroups indicated that human papillomavirus 16 infection might occur and play a potential role of progression in a minority of prostate cancer.