Estrogen prevents cellular senescence and bone loss through Usp10-dependent p53 degradation in osteocytes and osteoblasts: the role of estrogen in bone cell senescence

• Published in: Cell and tissue research Vol. 386; no. 2; pp. 297 - 308
• Main Authors: Wei, Yu, Fu, Jiayao, Wu, Wenjing, Ma, Pengfei, Ren, Le, Wu, Junhua
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.11.2021; Springer; Springer Nature B.V
• Subjects: Animals; Biomedical and Life Sciences; Biomedicine; Bone loss; Bone mass; Bones; Cell Line; Cellular Senescence; Cyclin-dependent kinase inhibitor p21; Degradation; Density; Estrogen; Estrogens; Estrogens - metabolism; Female; Fulvestrant; Homeostasis; Human Genetics; Humans; Hydrogen peroxide; Localization; Mice; Mice, Inbred C57BL; Molecular Medicine; Nuclear transport; Osteoblasts; Osteoblasts - cytology; Osteoblasts - metabolism; Osteocytes; Osteocytes - cytology; Osteocytes - metabolism; Osteoporosis, Postmenopausal - metabolism; Ovariectomy; p53 Protein; Phenols; Proteolysis; Proteomics; Regular Article; Senescence; Transcription; Tumor proteins; Tumor Suppressor Protein p53 - metabolism; Ubiquitin Thiolesterase - metabolism; β-Galactosidase; Usp10; Bone cells; Cellular senescence; Estrogen; p53
• ISSN: 0302-766X; 1432-0878
• DOI: 10.1007/s00441-021-03496-7

Estrogens play multiple roles in maintaining skeletal homeostasis by regulating many physiological processes in bone cells. Recently, cellular senescence in bone cells, especially in osteocytes, has been demonstrated to be a pivotal factor in bone loss. However, whether and how estrogen mediates cellular senescence in bone cells remains unknown. Here, we show that estrogen is negatively correlated with p53-related cellular senescence, primarily through the regulation of p53 protein levels, both in vivo and in vitro. Further study confirmed that estrogen attenuated the nuclear import of p53 and accelerated p53 degradation in osteocyte-like MLO-Y4 cells and osteoblastic MC3T3-E1 cells. A screen of p53-related ubiquitinating/deubiquitinating enzymes indicated that estrogen induced the degradation of p53 through the regulation of Usp10, a deubiquitinase that is directly linked to p53. Usp10 inhibition attenuated H 2 O 2 -induced senescence in MLO-Y4 cells, as indicated by p53/p21 quantification, a senescence-associated β-galactosidase (SA-β-gal) assay, and p53 localization visualization with a confocal microscope. Usp10 overexpression abolished the estrogen-mediated regulation of p53 and the downstream transcriptional gene p21. The injection of ovariectomized (OVX) mice with Spautin-1, a Usp10 inhibitor, inhibited the expression of p53 and the transcription of downstream senescence markers, as well as promoted bone mass recovery. Taken together, our study unveils the regulatory function of estrogen in the prevention of cellular senescence through the regulation of Usp10, thereby accelerating the degradation of senescent factor p53 and inhibiting its nuclear import.