Methylation of Promoters of Apoptosis-Related Genes in Blood Lymphocytes of Workers Exposed to Occupational External Irradiation

• Published in: Bulletin of experimental biology and medicine Vol. 171; no. 3; pp. 357 - 361
• Main Authors: Isubakova, D. S., Tsymbal, O. S., Bronikovskaya, E. V., Litviakov, N. V., Milto, I. V., Takhauov, R. М.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.07.2021; Springer; Springer Nature B.V
• Subjects: Aged; Analysis; Apoptosis; Apoptosis - genetics; Apoptosis Regulatory Proteins - genetics; Apoptosis Regulatory Proteins - metabolism; Apoptotic Protease-Activating Factor 1 - genetics; Apoptotic Protease-Activating Factor 1 - metabolism; bcl-2-Associated X Protein - genetics; bcl-2-Associated X Protein - metabolism; bcl-Associated Death Protein - genetics; bcl-Associated Death Protein - metabolism; BH3 Interacting Domain Death Agonist Protein - genetics; BH3 Interacting Domain Death Agonist Protein - metabolism; Biomedical and Life Sciences; Biomedicine; Cell Biology; Chromosome Aberrations - classification; Chromosome Aberrations - radiation effects; Chromosomes; DNA Methylation; Epigenesis, Genetic; Ethylenediaminetetraacetic acid; Gamma Rays - adverse effects; Genes; Genetics; Humans; Internal Medicine; Laboratory Medicine; Lymphocytes; Lymphocytes - metabolism; Lymphocytes - pathology; Lymphocytes - radiation effects; Male; Methylation; Middle Aged; Occupational Exposure - adverse effects; Pathology; Promoter Regions, Genetic; Radiation; Radiation Exposure - adverse effects; Radiometry; Repressor Proteins - genetics; Repressor Proteins - metabolism; Siberia; Tumor Suppressor Proteins - genetics; Tumor Suppressor Proteins - metabolism; Workers; γ Radiation; Siberia; DNA methylation; chromosomal aberrations; a promoter; apoptosis; ionizing radiation
• ISSN: 0007-4888; 1573-8221; 1573-8221
• DOI: 10.1007/s10517-021-05227-y

We studied the effect of technogenic radiation on the degree of promoter methylation in genes involved in apoptosis in blood lymphocytes of workers exposed to long-term γ-radiation during their professional activities. Blood samples for the analysis were obtained from 11 conventionally healthy men aged from 54 to 71 years (mean 66 years), workers of the Siberian Group of Chemical Enterprises working experience from 27 to 40 years (mean 30 years); the external exposure dose was 175.88 mSv (158.20-207.81 mSv). In all examined subjects, the degree of methylation of the promoters of apoptosis-related genes ranged from 0.22 to 50.00%. A correlation was found between the degree of methylation of BCLAF1 promoters ( p =0.035) with the age of workers, BAX promoters ( p =0.0289) with high content of aberrant cells, and APAF1 promoters ( p =0.0152) with increased number of dicentric chromosomes. A relationship was found between the dose of external irradiation and the degree of methylation of gene promoters of BAD ( p =0.0388), BID ( р =0.0426), and HRK ( р =0.0101) genes.