Microbial community regulation and performance enhancement in gas biofilters by interrupting bacterial communication

• Published in: Microbiome Vol. 10; no. 1; pp. 1 - 150
• Main Authors: Wang, Yong-Chao, Lin, Yu-Ting, Wang, Can, Tong, Zhen, Hu, Xu-Rui, Lv, Ya-Hui, Jiang, Guan-Yu, Han, Meng-Fei, Deng, Ji-Guang, Hsi, Hsing-Cheng, Lee, Chung-Hak
• Format: Journal Article
• Language: English
• Published: London BioMed Central 19.09.2022; BMC
• Subjects: Aromatic compounds; Bacteria; Bacterial communication; Bioassays; Biofilms; Biofilter; Biofilters; Biofiltration; Biomass; Bioreactors; Chemical communication; Enzymes; Function genes; Gene expression; Keystone species; Methods; Microbial community regulation; Microorganisms; Performance evaluation; Quorum quenching; Quorum sensing; Relative abundance; Rhodococcus; Sludge; VOCs; Volatile organic compounds; Water treatment
• ISSN: 2049-2618; 2049-2618
• DOI: 10.1186/s40168-022-01345-5

Abstract Background Controlling excess biomass accumulation and clogging is important for maintaining the performance of gas biofilters and reducing energy consumption. Interruption of bacterial communication (quorum quenching) can modulate gene expression and alter biofilm properties. However, whether the problem of excess biomass accumulation in gas biofilters can be addressed by interrupting bacterial communication remains unknown. Results In this study, parallel laboratory-scale gas biofilters were operated with Rhodococcus sp. BH4 (QQBF) and without Rhodococcus sp. BH4 (BF) to explore the effects of quorum quenching (QQ) bacteria on biomass accumulation and clogging. QQBF showed lower biomass accumulation (109 kg/m 3 ) and superior operational stability (85–96%) than BF (170 kg/m 3 ; 63–92%) at the end of the operation. Compared to BF, the QQBF biofilm had lower adhesion strength and decreased extracellular polymeric substance production, leading to easier detachment of biomass from filler surface into the leachate. Meanwhile, the relative abundance of quorum sensing (QS)-related species was found to decrease from 67 (BF) to 56% (QQBF). The QS function genes were also found a lower relative abundance in QQBF, compared with BF. Moreover, although both biofilters presented aromatic compounds removal performance, the keystone species in QQBF played an important role in maintaining biofilm stability, while the keystone species in BF exhibited great potential for biofilm formation. Finally, the possible influencing mechanism of Rhodococcus sp. BH4 on biofilm adhesion was demonstrated. Overall, the results of this study achieved excess biomass control while maintaining stable biofiltration performance (without interrupting operation) and greatly promoted the use of QQ technology in bioreactors. Graphical Abstract