Protective role of extracellular catalase (KatA) against UVA radiation in Pseudomonas aeruginosa biofilms

• Published in: Journal of photochemistry and photobiology. B, Biology Vol. 131; pp. 53 - 64
• Main Authors: Pezzoni, Magdalena, Pizarro, Ramón A., Costa, Cristina S.
• Format: Journal Article
• Language: English
• Published: Switzerland Elsevier B.V 05.02.2014
• Subjects: Animals; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biofilms; Biofilms - radiation effects; Catalase; Catalase - genetics; Catalase - metabolism; Cattle; Extracellular Space - enzymology; Genetic Complementation Test; KatA; Plankton; Pseudomonas; Pseudomonas aeruginosa; Pseudomonas aeruginosa - enzymology; Pseudomonas aeruginosa - physiology; Pseudomonas aeruginosa - radiation effects; Ultraviolet Rays; Ultraviolet-A; UVA; KatA; UVA; Biofilms; Catalase; Pseudomonas; Ultraviolet-A
• ISSN: 1011-1344; 1873-2682
• DOI: 10.1016/j.jphotobiol.2014.01.005

•KatA activity is present in the matrix of P. aeruginosa biofilms.•Extracellular KatA is essential in the defense of P. aeruginosa biofilms against UVA.•KatA is not involved in the resistance to photocatalysis of P. aeruginosa biofilms. One of the more stressful factors that Pseudomonas aeruginosa must face in nature is solar UVA radiation. In this study, the protective role of KatA catalase in both planktonic cells and biofilms of P. aeruginosa against UVA radiation was determined by using the wild-type (PAO1) and an isogenic catalase deficient strain (katA). The katA strain was more sensitive than the wild-type, especially in the case of biofilms. Moreover, the wild-type biofilm was more resistant than its planktonic counterpart, but this was not observed in the katA strain. Striking KatA activity was detected in the matrix of katA+ strains, and to our knowledge, this is the first report of this activity in the matrix of P. aeruginosa biofilms. Provision of bovine catalase or KatA to the matrix of a katA biofilm significantly increased its UVA tolerance, demonstrating that extracellular KatA is essential to optimal defense against UVA in P. aeruginosa biofilms. Efficiency of photocatalytic treatments using TiO2 and UVA was lower in biofilms than in planktonic cells, but KatA and KatB catalases seem not to be responsible for the higher resistance of the sessile cells to this treatment.