Heating or freezing bone: Effects on angiogenesis induction and growth potential in mice

We have characterized the effect of bone graft treatment by heating or freezing (with or without dimethyl sulfoxide (DMSO)). Tissue culture and dorsal skinfold chambers in mice were used as sites to quantify the effect on angiogenesis, growth and calcification of neonatal femora. Fresh femora increa...

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Bibliographic Details
Published inActa orthopaedica Vol. 67; no. 4; pp. 383 - 388
Main Authors Leunig, Michael, Yuan, Fan, Berk, David A, Gerweck, Leo E, Jain, Rakesh K
Format Journal Article
LanguageEnglish
Published England Informa UK Ltd 1996
Subjects
Animals
Animals, Newborn
Bone Transplantation - adverse effects
Culture Techniques
Femur - growth & development
Femur - physiology
Freezing
Heating
Mice
Mice, Nude
Neovascularization, Physiologic - physiology
Skin Window Technique
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Summary:We have characterized the effect of bone graft treatment by heating or freezing (with or without dimethyl sulfoxide (DMSO)). Tissue culture and dorsal skinfold chambers in mice were used as sites to quantify the effect on angiogenesis, growth and calcification of neonatal femora. Fresh femora increased in both length and cartilage diameter (calcification in vivo only), but cryopreservation or heating abolished the increase in femoral dimensions. in vivo, femora of all experimental groups elicited an angiogenic response from the host tissue, which was most pronounced for fresh femora, weaker for DMSO-pre-served frozen bone and poor for unprotected frozen bone and boiled femora. Freezing in the presence of a cryopreservative (DMSO) was found to preserve the angiogenic potential of frozen bone, whereas unprotected heating or freezing significantly impaired angiogenesis induction and growth potential.
ISSN:1745-3674
0001-6470
1745-3682
DOI:10.3109/17453679609002336