Perspective on fluorescence cell imaging with ionophore-based ion-selective nano-optodes

Inorganic ions are ubiquitous in all kinds of cells with highly dynamic spatial and temporal distribution. Taking advantage of different types of fluorescent probes, fluorescence microscopic imaging and quantitative analysis of ion concentrations in cells have rapidly advanced. A family of fluoresce...

Full description

Saved in:
Bibliographic Details
Published inBiomicrofluidics Vol. 16; no. 3; pp. 031301 - 31311
Main Authors Du, Xinfeng, Li, Niping, Chen, Qinghan, Wu, Zeying, Zhai, Jingying, Xie, Xiaojiang
Format Journal Article
LanguageEnglish
Published United States American Institute of Physics 01.05.2022
AIP Publishing LLC
Subjects
Calcium ions
Fluorescent indicators
Imaging
Temporal distribution
Online AccessGet full text

Cover

More Information
Summary:Inorganic ions are ubiquitous in all kinds of cells with highly dynamic spatial and temporal distribution. Taking advantage of different types of fluorescent probes, fluorescence microscopic imaging and quantitative analysis of ion concentrations in cells have rapidly advanced. A family of fluorescent nanoprobes based on ionophores has emerged in recent years with the potential to establish a unique platform for the analysis of common biological ions including Na+, K+, Ca2+, Cl−, and so on. This article aims at providing a retrospect and outlook of ionophore-based ion-selective nanoprobes and the applications in cell imaging.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1932-1058
1932-1058
DOI:10.1063/5.0090599