Polysomaty in Cymbidium

Nuclear DNA content in various parts of Cymbidium plants was measured by flow cytometry. Two types of Cymbidium, protocorm-like body (PLB)-propagated epiphytic hybrids and rhizome-propagated terrestrial C. kanran Makino demonstrated polysomaty. Small shoots on PLBs of Cymbidium hybrids showed two pe...

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Published inHortScience Vol. 37; no. 7; pp. 1088 - 1091
Main Authors Fukai, Seiichi, Hasegawa, Atsushi, Goi, Masanori
Format Journal Article
LanguageEnglish
Published Alexandria, VA American Society for Horticultural Science 01.12.2002
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Abstract Nuclear DNA content in various parts of Cymbidium plants was measured by flow cytometry. Two types of Cymbidium, protocorm-like body (PLB)-propagated epiphytic hybrids and rhizome-propagated terrestrial C. kanran Makino demonstrated polysomaty. Small shoots on PLBs of Cymbidium hybrids showed two peaks (2C and 4C) while PLBs showed four peaks, estimated to be 2C, 4C, 8C, and 16C. Roots and floral organs excluding ovaries of hybrids were highly polysomatic as were the rhizomes and roots of C. kanran. The patterns of polysomaty development were organ and developmental stage specific. Young leaves taken from in vitro plants are suitable material for determining the ploidy levels of Cymbidium plants by flow cytometry.
AbstractList Nuclear DNA content in various parts of Cymbidium plants was measured by flow cytometry. Two types of Cymbidium, protocorm-like body (PLB)-propagated epiphytic hybrids and rhizome-propagated terrestrial C. kanran Makino demonstrated polysomaty. Small shoots on PLBs of Cymbidium hybrids showed two peaks (2C and 4C) while PLBs showed four peaks, estimated to be 2C, 4C, 8C, and 16C. Roots and floral organs excluding ovaries of hybrids were highly polysomatic as were the rhizomes and roots of C. kanran. The patterns of polysomaty development were organ and developmental stage specific. Young leaves taken from in vitro plants are suitable material for determining the ploidy levels of Cymbidium plants by flow cytometry.
Author Fukai, Seiichi
Goi, Masanori
Hasegawa, Atsushi
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Snippet Nuclear DNA content in various parts of Cymbidium plants was measured by flow cytometry. Two types of Cymbidium, protocorm-like body (PLB)-propagated epiphytic...
Nuclear DNA content in various parts of Cymbidium plants was measured by flow cytometry. Two types of Cymbidium , protocorm-like body (PLB)-propagated...
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SubjectTerms Biological and medical sciences
Cymbidium
epiphytes
flow cytometry
Fundamental and applied biological sciences. Psychology
hybrids
leaves
nuclear genome
plant ovary
ploidy
rhizomes
roots
shoots
Title Polysomaty in Cymbidium
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