Polysomaty in Cymbidium
Nuclear DNA content in various parts of Cymbidium plants was measured by flow cytometry. Two types of Cymbidium, protocorm-like body (PLB)-propagated epiphytic hybrids and rhizome-propagated terrestrial C. kanran Makino demonstrated polysomaty. Small shoots on PLBs of Cymbidium hybrids showed two pe...
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Published in | HortScience Vol. 37; no. 7; pp. 1088 - 1091 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Alexandria, VA
American Society for Horticultural Science
01.12.2002
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Abstract | Nuclear DNA content in various parts of Cymbidium plants was measured by flow cytometry. Two types of Cymbidium, protocorm-like body (PLB)-propagated epiphytic hybrids and rhizome-propagated terrestrial C. kanran Makino demonstrated polysomaty. Small shoots on PLBs of Cymbidium hybrids showed two peaks (2C and 4C) while PLBs showed four peaks, estimated to be 2C, 4C, 8C, and 16C. Roots and floral organs excluding ovaries of hybrids were highly polysomatic as were the rhizomes and roots of C. kanran. The patterns of polysomaty development were organ and developmental stage specific. Young leaves taken from in vitro plants are suitable material for determining the ploidy levels of Cymbidium plants by flow cytometry. |
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AbstractList | Nuclear DNA content in various parts of Cymbidium plants was measured by flow cytometry. Two types of Cymbidium, protocorm-like body (PLB)-propagated epiphytic hybrids and rhizome-propagated terrestrial C. kanran Makino demonstrated polysomaty. Small shoots on PLBs of Cymbidium hybrids showed two peaks (2C and 4C) while PLBs showed four peaks, estimated to be 2C, 4C, 8C, and 16C. Roots and floral organs excluding ovaries of hybrids were highly polysomatic as were the rhizomes and roots of C. kanran. The patterns of polysomaty development were organ and developmental stage specific. Young leaves taken from in vitro plants are suitable material for determining the ploidy levels of Cymbidium plants by flow cytometry. |
Author | Fukai, Seiichi Goi, Masanori Hasegawa, Atsushi |
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Snippet | Nuclear DNA content in various parts of Cymbidium plants was measured by flow cytometry. Two types of Cymbidium, protocorm-like body (PLB)-propagated epiphytic... Nuclear DNA content in various parts of Cymbidium plants was measured by flow cytometry. Two types of Cymbidium , protocorm-like body (PLB)-propagated... |
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SubjectTerms | Biological and medical sciences Cymbidium epiphytes flow cytometry Fundamental and applied biological sciences. Psychology hybrids leaves nuclear genome plant ovary ploidy rhizomes roots shoots |
Title | Polysomaty in Cymbidium |
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