Polysomaty in Cymbidium

Nuclear DNA content in various parts of Cymbidium plants was measured by flow cytometry. Two types of Cymbidium, protocorm-like body (PLB)-propagated epiphytic hybrids and rhizome-propagated terrestrial C. kanran Makino demonstrated polysomaty. Small shoots on PLBs of Cymbidium hybrids showed two pe...

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Bibliographic Details
Published inHortScience Vol. 37; no. 7; pp. 1088 - 1091
Main Authors Fukai, Seiichi, Hasegawa, Atsushi, Goi, Masanori
Format Journal Article
LanguageEnglish
Published Alexandria, VA American Society for Horticultural Science 01.12.2002
Subjects
Biological and medical sciences
Cymbidium
epiphytes
flow cytometry
Fundamental and applied biological sciences. Psychology
hybrids
leaves
nuclear genome
plant ovary
ploidy
rhizomes
roots
shoots
flow cytometry
polysomaty
Cymbidium
orchid
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Summary:Nuclear DNA content in various parts of Cymbidium plants was measured by flow cytometry. Two types of Cymbidium, protocorm-like body (PLB)-propagated epiphytic hybrids and rhizome-propagated terrestrial C. kanran Makino demonstrated polysomaty. Small shoots on PLBs of Cymbidium hybrids showed two peaks (2C and 4C) while PLBs showed four peaks, estimated to be 2C, 4C, 8C, and 16C. Roots and floral organs excluding ovaries of hybrids were highly polysomatic as were the rhizomes and roots of C. kanran. The patterns of polysomaty development were organ and developmental stage specific. Young leaves taken from in vitro plants are suitable material for determining the ploidy levels of Cymbidium plants by flow cytometry.
Bibliography:http://hortsci.ashspublications.org/content/37/7.toc
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ISSN:0018-5345
2327-9834
DOI:10.21273/hortsci.37.7.1088