Culture conditions and medium components for the production of mycelial biomass and exo-polysaccharides with Paecilomyces japonica in liquid culture

• Published in: Journal of bioscience and bioengineering Vol. 115; no. 4; pp. 433 - 437
• Main Authors: Lee, Jong Seok, Jung, Woo Chul, Park, Seok Jae, Lee, Keun Eok, Shin, Won Cheol, Hong, Eock Kee
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.04.2013; Elsevier
• Subjects: Aeration; agitation; Biological and medical sciences; Biomass; Biotechnology; carbon; Carbon - metabolism; carbon nitrogen ratio; Carbon-to-nitrogen ratio; Culture Media - chemistry; Entomopathogenic fungus; Exo-polysaccharide; Fundamental and applied biological sciences. Psychology; glucose; Hydrogen-Ion Concentration; Mycelial growth; mycelium; Mycelium - growth & development; nitrogen; Nitrogen - metabolism; Paecilomyces; Paecilomyces - growth & development; Paecilomyces - metabolism; Paecilomyces japonica; polysaccharides; Polysaccharides, Bacterial - biosynthesis; Polysaccharides, Bacterial - chemistry; potassium dihydrogen phosphate; Temperature; Trace Elements - metabolism; yeast extract; Mycelial growth; Entomopathogenic fungus; Carbon-to-nitrogen ratio; Exo-polysaccharide; Paecilomyces japonica; Culture medium; Entomopathogen; Growth; Biomass; Fungi; Carbon nitrogen ratio; Paecilomyces; Production; Fungi Imperfecti; Polysaccharide; Culture
• ISSN: 1389-1723; 1347-4421
• DOI: 10.1016/j.jbiosc.2012.10.022

In this study, the liquid culture conditions were optimized for maximal production of mycelial biomass and exo-polysaccharide by Paecilomyces japonica. The effects of medium composition, C/N ratio and physical parameters were investigated. From these experiments, 30 g glucose, 20 g yeast extract, 0.5 g KH2PO4, and 0.1 g CuCl2 2H2O in 1-l distilled water were found to be the most suitable carbon, nitrogen, and mineral sources, respectively. The optimal temperature, initial pH, agitation, and aeration were determined to be 27°C, uncontrolled pH, 400 rpm, and 1.0 vvm, respectively. Under these optimal conditions, the maximum mycelial growth and polysaccharides production were 23.1 g/l and 2.5 g/l, respectively.