The stability, toxicity and effectiveness of unmodified and phosphorothioate antisense oligodeoxynucleotides in Xenopus oocytes and embryos

• Published in: Nucleic acids research Vol. 18; no. 7; pp. 1763 - 1769
• Main Authors: WOOLF, T. M, JENNINGS, C. G. B, REBAGLIATI, M, MELTON, D. A
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 11.04.1990
• Subjects: Animals; Base Sequence; Biological and medical sciences; Biotechnology; Cell Survival - drug effects; Embryo, Nonmammalian - cytology; Embryo, Nonmammalian - drug effects; Embryo, Nonmammalian - metabolism; Endoribonucleases - metabolism; Female; Fundamental and applied biological sciences. Psychology; Genetic engineering; Genetic technics; Methods. Procedures. Technologies; Modification of gene expression level; Molecular Sequence Data; Oligonucleotides - chemical synthesis; Oligonucleotides - metabolism; Oligonucleotides - toxicity; Oligonucleotides, Antisense; Oocytes - cytology; Oocytes - drug effects; Oocytes - metabolism; Ribonuclease H; RNA - genetics; RNA - isolation & purification; RNA, Messenger - genetics; Thionucleotides - chemical synthesis; Thionucleotides - metabolism; Thionucleotides - toxicity; Xenopus; Vertebrata; Embryo; Messenger RNA; Enzyme; Xenopus laevis; Amphibia; Salientia; Genetic engineering; Ribonuclease; Gene expression; Oligodeoxyribonucleotide; Oocyte
• ISSN: 0305-1048; 1362-4962
• DOI: 10.1093/nar/18.7.1763

The properties of antisense phosphorothioate and unmodified oligodeoxynucleotides have been studied in Xenopus oocytes and embryos. We find that phosphorothioates, like unmodified oligodeoxynucleotides, can degrade Vg1 mRNA in oocytes via an endogenous RNase H-like activity. In oocytes, phosphorothioate oligodeoxynucleotides are more stable than unmodified oligodeoxynucleotides and are more effective in degrading Vg1 mRNA. In embryos, neither unmodified nor phosphorothioate deoxyoligonucleotides were effective in degrading Vg1 message at sub-toxic doses.