A recombinant fowlpox virus expressing the hemagglutinin-neuraminidase gene of Newcastle disease virus (NDV) protects chickens against challenge NDV

• Published in: Virology (New York, N.Y.) Vol. 178; no. 1; pp. 297 - 300
• Main Authors: Boursnell, M.E.G., Green, P.F., Samson, A.C.R., Campbell, J.I.A., Deuter, A., Peters, R.W., Millar, N.S., Emmerson, P.T., Binns, M.M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.09.1990
• Subjects: AGGLUTININE; AGGLUTININS; AGLUTININAS; Animals; Antibodies, Monoclonal - immunology; AVES DE CORRAL; AVIPOXVIRUS; Chickens - genetics; Chickens - immunology; Chickens - microbiology; Cloning, Molecular; Culture Techniques; EXPRESION GENICA; EXPRESSION DES GENES; Fowlpox virus - genetics; GENE; GENE EXPRESSION; GENE TRANSFER; GENES; Genes, Viral; Genetic Vectors; GLICOSIDASAS; GLYCOSIDASE; GLYCOSIDASES; HN Protein - biosynthesis; HN Protein - genetics; IMMUNITE; IMMUNITY; INMUNIDAD; NEWCASTLE DISEASE VIRUS; Newcastle disease virus - enzymology; Newcastle disease virus - genetics; POULTRY; Promoter Regions, Genetic; RECOMBINACION; RECOMBINAISON; RECOMBINANT VACCINES; RECOMBINATION; Recombination, Genetic; SIALIDASE; STRAINS; SYNTHETIC VACCINES; TRANSFERENCIA DE GENES; TRANSFERT DE GENE; VACCIN SYNTHETIQUE; VACUNA SINTETICA; VECTEUR DE MALADIE; VECTORES; VECTORS; Viral Vaccines; VIRUS DE LA ENFERMEDAD DE NEWCASTLE; VIRUS MALADIE DE NEWCASTLE; VOLAILLE
• ISSN: 0042-6822; 1096-0341
• DOI: 10.1016/0042-6822(90)90408-J

The hemagglutinin-neuraminidase (HN) gene from the Beaudette C strain of Newcastle disease virus (NDV) has been expressed in a recombinant fowlpox virus vector. The HN gene, under the control of the vaccinia p7.5 promoter, was inserted into a nonessential gene in the terminal inverted repeats of fowlpox virus. Expression was demonstrated in tissue culture, a protein of the correct size for fully glycosylated HN protein being recognized by an HN-specific monoclonal antibody on Western blots. When the recombinant fowlpox virus was inoculated into chickens by intravenous or wing-web routes, antibody which recognizes HN from purified NDV virions wasinoculated. Protective immunity to NDV was generated in the chickens; at the highest dose of vaccine 100% of the chickens tested were protected against challenge with a virulent strain of NDV.