Molecular cloning and characterization of pathogenesis-related protein family 10 gene from spinach (SoPR10)

• Published in: Bioscience, biotechnology, and biochemistry Vol. 78; no. 5; pp. 780 - 786
• Main Authors: Bai, Xuegui, Long, Juan, He, Xiaozhao, Li, Shun, Xu, Huini
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 2014; Oxford University Press
• Subjects: Amino Acid Sequence; Base Sequence; Cloning, Molecular; expression profiles; Gene Expression Regulation, Plant - drug effects; Intracellular Space - drug effects; Intracellular Space - metabolism; Models, Molecular; Molecular Sequence Data; nitrate stress; Nitrates - pharmacology; pathogenesis-related protein; Plant Proteins - chemistry; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Roots - drug effects; Plant Roots - metabolism; Protein Conformation; Protein Transport - drug effects; Sequence Analysis; spinach; Spinacia oleracea - cytology; Spinacia oleracea - drug effects; Spinacia oleracea - genetics; Spinacia oleracea - physiology; Stress, Physiological - drug effects; nitrate stress; spinach; pathogenesis-related protein; expression profiles
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1080/09168451.2014.910094

PR10 genes encode small, intracellular proteins that respond to biotic and abiotic stresses. In this study, a cDNA clone (designated as SoPR10, GenBank Accession No. KC142174) encoding a PR10 protein from spinach (Spinacia oleracea L.) was isolated and characterized. SoPR10 encoded a 161-amino acid polypeptide with a predicted molecular mass of 19.76 kDa and a pI of 4.61. Real-time quantitative analysis indicated that SoPR10 was constitutively expressed in root and shoot. The abundance of SoPR10 in salt-resistant cultivar (Chaoji) was generally greater than in salt-sensitive cultivar (Daye) under 160 mM L −1 treatment for 0.5, 3, and 6 h. The expression of SoPR10 was also induced by other abiotic stresses including polyethylene glycol, NaCl, salicylic acid, and H 2 O 2 . Our results indicated that SoPR10 might play important roles under nitrate stress and other abiotic stresses. PR10 genes encode small, intracellular proteins that respond to biotic and abiotic stresses.