Effect of Probiotic Lacticaseibacillus rhamnosus LB1.5 on Anxiety-like Behavior, Neuroprotection and Neuroinflammation Markers of Male Mice Fed a High-Fat Diet

• Published in: Nutrients Vol. 16; no. 6; p. 879
• Main Authors: Schmidt, Natália Perin, Molz, Patrícia, Fraga, Brenda Santos, Bondarczuk, Nicole Hiller, Silveira, Priscila Dutra, Ferri, Milena Henrique, Crestani, Thais Busatto, Breyer, Gabriela Merker, Guimarães, Giuliano Rizzoto, Motta, Amanda de Souza da, Guedes, Renata Padilha, Giovenardi, Márcia
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 18.03.2024; MDPI
• Subjects: Animals; Anxiety; Anxiety - prevention & control; anxiety-like behavior; Bdnf; Cytokines; Diet; Diet, High-Fat - adverse effects; DOHaD; Gene expression; Interleukin-6; International organizations; Laboratory animals; Lacticaseibacillus rhamnosus; Light; Male; Males; Mice; Mice, Inbred C57BL; Neuroinflammatory Diseases; Neuroprotection; Nrf2; Obesity; Probiotics; Sirt1; Tumor necrosis factor-TNF; Brazil; United States > US; Sirt1; DOHaD; anxiety-like behavior; Bdnf; Nrf2
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu16060879

Probiotic supplementation has been identified as a potential target to reduce inflammatory mediators associated with obesity. Therefore, this study assessed the effect of probiotic LB1.5 on anxiety-like behavior, gene expression in the prefrontal cortex, and neuroinflammation in the cerebral cortex and hippocampus of male mice fed a high-fat diet. Mice aged 21 days were divided into four groups: control (CONT), control plus probiotic (CONT + PROB), high-fat diet (HFD), and high-fat diet plus probiotic (HFD + PROB), and fed for 13 weeks. The probiotic 1.5 (3.1 × 108 CFU/mL, derived from raw buffalo milk) was administered by gavage three times a week. Probiotic supplementation provided an anxiolytic effect in CONT and HFD. The IL-6 showed lower levels after probiotic supplementation in the HFD. Regarding immunoreactivity for GFAP in the cerebral cortex, we demonstrated that animals HFD-fed had a reduction in cells number compared to CONT. In the hippocampus, we found an interaction between diet and supplementation, as well as an effect of probiotic supplementation. A higher number of Th positive cells was observed in the cerebral cortex in mice fed HFD. LB1.5 supplementation decreased serum IL-6 levels in HFD-fed mice and promoted a reduction in anxiety-like behavior.