An original class of small sized molecules as versatile fluorescent probes for cellular imaging

• Published in: Chemical communications (Cambridge, England) Vol. 55; no. 54; pp. 7776 - 7779
• Main Authors: Sirbu, Doina, Diharce, Julien, Martini, Ivana, Chopin, Nicolas, Eliseeva, Svetlana V, Guillaumet, Gérald, Petoud, Stéphane, Bonnet, Pascal, Suzenet, Franck
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 02.07.2019
• Subjects: Chemical Sciences; Fluorescence; Fluorescent Dyes - chemical synthesis; Fluorescent Dyes - chemistry; Fluorescent indicators; HeLa Cells; Heterocyclic Compounds, 3-Ring - chemical synthesis; Heterocyclic Compounds, 3-Ring - chemistry; Humans; Imaging; Microscopy, Fluorescence - methods; NMR; Nuclear magnetic resonance; Optical Imaging - methods; Organic chemistry; Photobleaching; Toxicity; spectroscopic analysis; cytotoxicity tests See; Electronic Supplementary Information (ESI) available: Experimental procedures; characterization data; theoretical calculations; 1 H and 13 C NMR spectra; cellular optical imaging
• ISSN: 1359-7345; 1364-548X
• DOI: 10.1039/c9cc03765a

An unusual class, compact in size, of fluorescent probes based on pyridazino-1,3 a ,6 a -triazapentalene scaffolds exhibits promising fluorescent properties (quantum yield values up to 73%, large Stokes shifts, emission wavelengths located in the green-yellow range, excellent solubility) with good photostability suitable for optical imaging applications. An unusual class, compact in sizes, of fluorescent probes based on pyridazino-1,3 a ,6 a -triazapentalene scaffolds exhibits promising fluorescent properties with good solubility and good photostability suitable for optical imaging applications.