Fabrication, characterization and potential application of biodegradable polydopamine-modified scaffolds based on natural macromolecules

• Published in: International Journal of Biological Macromolecules Vol. 253; no. Pt 1; p. 126596
• Main Authors: Wang, Yiyu, Wang, Xinyu, Liu, Xingxun, Niu, Chunqing, Yu, Guiting, Hou, Yuanjing, Hu, Chao, Zhao, Kai, Shi, Jian
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 31.12.2023; Elsevier BV
• Subjects: Biodegradability; collagen; cytotoxicity; Dihydroxyphenylalanine; drugs; Fibroins; hemolysis; imines; oxidation; Oxidized sodium alginate; Polydopamine; Polymers; Scaffold; sodium alginate; Surface modification; thermal stability; Tissue Engineering; Tissue Scaffolds; Polydopamine; Oxidized sodium alginate; Surface modification; Scaffold; Biodegradability
• ISSN: 0141-8130; 1879-0003; 1879-0003
• DOI: 10.1016/j.ijbiomac.2023.126596

Sodium alginate (SA)-based implantable scaffolds with slow-release drugs have become increasingly important in the fields of biomedical and tissue engineering. However, high-molecular-weight SA is difficult to remove from the body due to the lack of SA-degrading enzymes. The very slow degradation properties of SA-based scaffolds limit their applications. Herein, we designed a series of biodegradable oxidized SA (OSA)-based scaffolds through amide bonds, imine bonds and hydrogen bridges between OSA and silk fibroin (SF). SF/OSA-0.4 with a blend ratio of 4/1 was chosen for further polydopamine (PDA) surface modification studies through the optimization of those parameters such as different OSA oxidation degrees, and blend ratios. PDA modified SF/OSA-0.4 (Dopa/SF/OSA-0.4) showed the excellent stability, better stretchable properties, a uniform interconnective porous structure, high thermal stability, a low hemolysis ratio and cytotoxicity. In vitro degradation experiments showed that the degradation rate of SF/OSA was significantly higher than that of SF/SA, but the degradation slowed again after PDA modification. Interestingly, the degradation of Dopa/SF/OSA-0.4 in vivo was significantly faster than that in vitro. Dopa/SF/OSA-0.4 was also more conducive to new tissue growth and collagen bundle formation. Moreover, Dopa/SF/OSA-0.4 improved the absorbability of RhB (model drug) and reduced the sudden release of RhB during the sustained release. A novel stabilized SF/OSA-0.4 scaffold coated with PDA was optimized. The Dopa/SF/OSA-0.4 scaffold can be biodegraded in vivo and in vivo, and exhibited better versatility for new tissue growth in vivo. Moreover, it can absorb more RhB (model drug) and reduce the sudden release of RhB. This study provides an innovative option for biodegradable implant materials with sustained drug release. [Display omitted]