Caffeic acid ameliorates early and delayed brain injuries after focal cerebral ischemia in rats

• Published in: Acta pharmacologica Sinica Vol. 27; no. 9; pp. 1103 - 1110
• Main Authors: Zhou, Yu, Fang, San-hua, Ye, Yi-lu, Chu, Li-sheng, Zhang, Wei-ping, Wang, Meng-ling, Wei, Er-qing
• Format: Journal Article
• Language: English
• Published: United States Nature Publishing Group 01.09.2006
• Subjects: Animals; Antioxidants - pharmacology; Arachidonate 5-Lipoxygenase - metabolism; Brain - pathology; Caffeic Acids - pharmacology; Cysteine - metabolism; Infarction, Middle Cerebral Artery - complications; Ischemic Attack, Transient - etiology; Ischemic Attack, Transient - metabolism; Ischemic Attack, Transient - pathology; Leukotriene B4 - metabolism; Leukotrienes - metabolism; Male; Neurons - drug effects; Neuroprotective Agents - pharmacology; Rats; Rats, Sprague-Dawley; 动物实验; 咖啡酸; 脑损伤; 脑缺血
• ISSN: 1671-4083; 1745-7254
• DOI: 10.1111/j.1745-7254.2006.00406.x

Aim： To investigate the effects of caffeic acid on early and delayed injuries after focal cerebral ischemia in rats, and the possible relation to 5-1ipoxygenase inhibition. Methods： Transient focal cerebral ischemia was induced by middle cerebral artery occlusion in Sprague-Dawley rats. Caffeic acid （10 and 50 mg/kg） was ip injected for 5 d after ischemia. The brain injuries were observed, and the levels of cysteinyl leukotrienes and leukotriene B4 in the brain tissue were measured. Results： Caffeic acid （50 mg/kg） ameliorated neurological dysfunction and neuron loss, and decreased infarct volume 24 h after ischemia; it attenuated brain atrophy, infarct volume, and particularly astrocyte proliferation 14 d after ischemia. In addition, it reduced the production of leukotrienes （5-1ipoxygenase metabolites） in the ischemic hemispheres 3 h and 7 d after ischemia. Conclusion： Caffeic acid has protective effect on both early and delayed injuries after focal cerebral ischemia in rats; and this effect may partly relate to 5-1ipoxygenase inhibition.