Comparison of the analgesic effects of various opioid peptides by a newly devised intracisternal injection technique in conscious mice

To determine accurately the central analgesic effects of opioid peptides, we devised a special technique for intracisternal administration of drugs to conscious mice. When this method was utilized we found that a series of endogenous opioid peptides and enkephalin analogues, (D-Ala2,Met5)-enkephalin...

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Bibliographic Details
Published inEuropean journal of pharmacology Vol. 56; no. 3; p. 265
Main Authors Ueda, H, Amano, H, Shiomi, H, Takagi, H
Format Journal Article
LanguageEnglish
Published Netherlands 15.06.1979
Subjects
Animals
Cisterna Magna
Endorphins - administration & dosage
Endorphins - pharmacology
Enkephalins - pharmacology
Injections, Spinal - methods
Male
Mice
Morphine - pharmacology
Motor Activity - drug effects
Naloxone - pharmacology
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Summary:To determine accurately the central analgesic effects of opioid peptides, we devised a special technique for intracisternal administration of drugs to conscious mice. When this method was utilized we found that a series of endogenous opioid peptides and enkephalin analogues, (D-Ala2,Met5)-enkephalinamide and (D-Met2,Pro5)-enkephalinamide produced dose-related analgesic effects, as determined by the tail pinch test. These effects were antagonized by pretreatment with naloxone 0.5 mg/kg s.c. This technique should be a most accurate one for the determination of central analgesic effects of various drugs in conscious mice.
ISSN:0014-2999
DOI:10.1016/0014-2999(79)90181-X