LncRNA SNHG14 promotes OGD/R-induced neuron injury by inducing excessive mitophagy via miR-182-5p/BINP3 axis in HT22 mouse hippocampal neuronal cells

• Published in: Biological research Vol. 53; no. 1; pp. 1 - 11
• Main Authors: Deng, Zexiang, Ou, Hao, Ren, Fei, Guan, Yujiao, Huan, Ye, Cai, Hongwei, Sun, Bei
• Format: Journal Article
• Language: English
• Published: Santiago BioMed Central 10.09.2020; BMC
• Subjects: Antibodies; Apoptosis; Autophagy; Binding sites; BINP3; BNIP3 protein; Caspase-3; Caspase-9; Cerebral ischemia–reperfusion injury; Flow cytometry; Glucose; Hippocampus; Ischemia; miR-182-5p; Mitochondria; Mitophagy; Non-coding RNA; Nucleoli; Oxidative stress; Proteins; Reagents; Reperfusion; SNHG14; snoRNA; United States > US; China
• ISSN: 0717-6287; 0716-9760; 0717-6287
• DOI: 10.1186/s40659-020-00304-4

Background Long non-coding RNA (lncRNA) small nucleolar RNA host gene 14 (SNHG14) is associated with cerebral ischemia–reperfusion (CI/R) injury. This work aims to explore the role of SNHG14 in CI/R injury. Methods HT22 (mouse hippocampal neuronal cells) cell model was established by oxygen–glucose deprivation/reoxygenation (OGD/R) treatment. The interaction among SNHG14, miR-182-5p and BNIP3 was verified by luciferase reporter assay. Flow cytometry, western blot and quantitative real-time PCR were performed to examine apoptosis, the expression of genes and proteins. Results SNHG14 and BNIP3 were highly expressed, and miR-182-5p was down-regulated in the OGD/R-induced HT22 cells. OGD/R-induced HT22 cells exhibited an increase in apoptosis. SNHG14 overexpression promoted apoptosis and the expression of cleaved-caspase-3 and cleaved-caspase-9 in the OGD/R-induced HT22 cells. Moreover, SNHG14 up-regulation enhanced the expression of BNIP3, Beclin-1, and LC3II/LC3I in the OGD/R-induced HT22 cells. Furthermore, SNHG14 regulated BNIP3 expression by sponging miR-182-5p. MiR-182-5p overexpression or BNIP3 knockdown repressed apoptosis in OGD/R-induced HT22 cells, which was abolished by SNHG14 up-regulation. Conclusion Our study demonstrates that lncRNA SNHG14 promotes OGD/R-induced neuron injury by inducing excessive mitophagy via miR-182-5p/BINP3 axis in HT22 mouse hippocampal neuronal cells. Thus, SNHG14/miR-182-5p/BINP3 axis may be a valuable target for CI/R injury therapies.