Chemical Compositions and Experimental and Computational Modeling of the Anticancer Effects of Cnidocyte Venoms of Jellyfish Cassiopea andromeda and Catostylus mosaicus on Human Adenocarcinoma A549 Cells

• Published in: Marine drugs Vol. 21; no. 3; p. 168
• Main Authors: Zare, Afshin, Afshar, Alireza, Khoradmehr, Arezoo, Baghban, Neda, Mohebbi, Gholamhossein, Barmak, Alireza, Daneshi, Adel, Bargahi, Afshar, Nabipour, Iraj, Almasi-Turk, Sahar, Arandian, Alireza, Zibaii, Mohammad Ismail, Latifi, Hamid, Tamadon, Amin
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 07.03.2023; MDPI
• Subjects: A549 Cells; Adenocarcinoma; Adenocarcinoma - drug therapy; Animals; Anticancer properties; Antimicrobial agents; Antitumor agents; Apoptosis; Biological activity; Biological effects; Cancer; Cassiopea andromeda; Catostylus mosaicus; Cells; Chemical composition; Cnidaria; Cnidarian Venoms - chemistry; Cnidarian Venoms - pharmacology; Computer applications; Cytotoxicity; Death receptors; Fatalities; Humans; Inflammation; jellyfish; Lung cancer; Lung Neoplasms - drug therapy; Marine animals; Marine environment; Marine invertebrates; Marine organisms; Medical prognosis; Molecular docking; Molecular Docking Simulation; Molecular dynamics; Natural resources; Oceans; Proteins; pulmonary adenocarcinoma; Scyphozoa; Venom; Persian Gulf; apoptosis; molecular docking; venom; jellyfish; pulmonary adenocarcinoma
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md21030168

Nowadays, major attention is being paid to curing different types of cancers and is focused on natural resources, including oceans and marine environments. Jellyfish are marine animals with the ability to utilize their venom in order to both feed and defend. Prior studies have displayed the anticancer capabilities of various jellyfish. Hence, we examined the anticancer features of the venom of and in an in vitro situation against the human pulmonary adenocarcinoma (A549) cancer cell line. The MTT assay demonstrated that both mentioned venoms have anti-tumoral ability in a dose-dependent manner. Western blot analysis proved that both venoms can increase some pro-apoptotic factors and reduce some anti-apoptotic molecules that lead to the inducing of apoptosis in A549 cells. GC/MS analysis demonstrated some compounds with biological effects, including anti-inflammatory, antioxidant and anti-cancer activities. Molecular docking and molecular dynamic showed the best position of each biologically active component on the different death receptors, which are involved in the process of apoptosis in A549 cells. Ultimately, this study has proven that both venoms of and have the capability to suppress A549 cells in an in vitro condition and they might be utilized in order to design and develop brand new anticancer agents in the near future.