MicroRNA-34a inhibits tumor invasion and metastasis in osteosarcoma partly by effecting C-IAP2 and Bcl-2

• Published in: Tumor biology Vol. 39; no. 6; p. 101042831770576
• Main Authors: Wen, Jie, Zhao, Yan-kun, Liu, Yan, Zhao, Jin-feng
• Format: Journal Article
• Language: English
• Published: London, England SAGE Publications 01.06.2017; Sage Publications Ltd; SAGE Publishing
• Subjects: Adolescents; Animal research; Animals; Annexin V; Apoptosis; Apoptosis - genetics; Authorship; Bcl-2 protein; Biochemistry; Bone cancer; Bone tumors; Caspase; Caspase 3 - genetics; Caspase 9 - genetics; Caspase-3; Caspase-9; Cell Movement - genetics; Cell Proliferation - genetics; Chemotherapy; Children; Fluorescein isothiocyanate; Gene Expression Regulation, Neoplastic; Genes; Humans; Inhibitor of Apoptosis Proteins - biosynthesis; Inhibitor of Apoptosis Proteins - genetics; Medical prognosis; Metastases; Metastasis; Mice; MicroRNAs; MicroRNAs - biosynthesis; MicroRNAs - genetics; miRNA; mRNA; Neoplasm Invasiveness - genetics; Neoplasm Invasiveness - pathology; Neoplasm Metastasis; Osteosarcoma; Osteosarcoma - genetics; Osteosarcoma - pathology; Osteosarcoma cells; Polymerase chain reaction; Proteins; Proto-Oncogene Proteins c-bcl-2 - biosynthesis; Proto-Oncogene Proteins c-bcl-2 - genetics; Radiation therapy; Sarcoma; Skin; Spectrophotometry; Xenograft Model Antitumor Assays; United States > US; China; Bcl-2; apoptosis; Osteosarcoma; miR-34a; C-IAP2; caspase
• ISSN: 1010-4283; 1423-0380; 1423-0380
• DOI: 10.1177/1010428317705761

Osteosarcoma is a common primary malignant bone tumor that occurs mainly in children and adolescents. Recent evidence has demonstrated that miR-34a is involved in the invasion and metastasis of osteosarcoma. This study aims to explore the effect of biological behavior of miR-34a on osteosarcoma. First, we collect osteosarcoma and adjacent specimens, and the relative expression of miR-34a and C-IAP2 messenger RNA was quantitated by real-time polymerase chain reaction. Furthermore, miR-34a stimulant is synthesized and transfected onto osteosarcoma MG-63 cells. The effect of overexpression of miR-34a on osteosarcoma was detected by colony-forming assay, Annexin V-fluorescein isothiocyanate Apoptosis Detection Kit I, Transwell assay, and animal experiment in vivo. Finally, the relative levels of C-IAP2 and Bcl-2 protein were checked by western blot, and the activity of caspase-3 and caspase-9 was tested by spectrophotometry assay. In conclusion, miR-34a was downregulated in osteosarcoma cells. And the expression of C-IAP2 and Bcl-2 protein was drastically inhibited, and the activities of caspase-3 and caspase-9 were significantly increased after transfecting miR-34a onto osteosarcoma MG-63 cells. And the overexpression of miR-34a can inhibit cell invasion and metastasis, promote cell apoptosis, and arrest cells in G0/G1 period. And the animal experiment in vivo demonstrated that the overexpression of miR-34a could significantly inhibit the growth of osteosarcoma in animal skin. Taken together, we indicated that miR-34a can inhibit tumor invasion and metastasis in osteosarcoma, and its mechanism may be partly related to downregulating the expression of C-IAP2 and Bcl-2 protein directly or indirectly.