Low-intensity Treadmill Exercise Promotes Rat Dorsal Wound Healing

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 36; no. 1; pp. 121 - 126
• Main Author: 周武 刘国辉 杨述华 米博斌 叶树楠
• Format: Journal Article
• Language: English
• Published: Wuhan Huazhong University of Science and Technology 01.02.2016; Department of Orthopedics,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China%Department of Orthopedics,Fourth Military Medical College Affiliated Tangdu Hospital,Xi'an 710032,China
• Subjects: Actins - metabolism; Animals; Collagen Type III - metabolism; Cytokines - blood; Endothelial Progenitor Cells - cytology; Male; Medicine; Medicine & Public Health; Nitric Oxide Synthase Type III - blood; Physical Exertion; Rats; Rats, Sprague-Dawley; Receptor, TIE-1 - metabolism; RNA, Messenger - blood; Running; Vascular Endothelial Growth Factor A - blood; Vascular Endothelial Growth Factor Receptor-2 - blood; Wound Healing; 临床; 医学; 症状; 诊断; wound healing; treadmill exercise; endothelial progenitor cells; vascular endothelial growth factor; angiopoietin
• ISSN: 1672-0733; 1993-1352; 1993-1352
• DOI: 10.1007/s11596-016-1553-3

In order to investigate the promoting effect of low-intensity treadmill exercise on rat dorsal wound healing and the mechanism, 20 Sprague-Dawley rats were randomly divided into two groups： exercise group（Ex） and non-exercise group（non-ex）. The rats in Ex group were given treadmill exercise for one month, and those in non-ex group raised on the same conditions without treadmill exercise. Both groups received dorsal wound operation with free access to food and water. By two-week continuous observation and recording of the wound area, the healing rate was analyzed. The blood sample was collected at day 14 post-operation via cardiac puncture for determination of the number of endothelial progenitor cells（EPCs） by flow cytometry, and the concentrations of relevant cytokines such as basic fibroblast growth factor（b FGF）, endothelial nitric oxide synthase（e NOS） and vascular endothelial growth factor（VEGF） were measured by ELISA. The skin tissue around the wound was dissected to observe the vascular density under the microscope after HE staining, to detect the m RNA level of VEGFR2 and angiopoietin-1（Ang-1） receptor using RT-q PCR, and protein expression of α-smooth muscle actin（αSMA） and type collagenⅢ（ColⅢ） using Western blotting. It was found that the wound area in Ex group was smaller at the same time point than in non-ex group. The number of circulating EPCs was greater and the concentrations of vasoactive factors such as VEGF, e NOS and b FGF were higher in Ex group than in non-ex group. HE staining displayed a higher vessel density in Ex group than in non-ex group. Moreover, the m RNA expression of VEGFR2 and Ang-1 detected in the wound tissue in Ex group was higher than in non-ex group. Meanwhile, the protein expression of αSMA and Col Ⅲwas more abundant in Ex group than in non-ex group. Conclusively, the above results demonstrate Ex rats had a higher wound healing rate, suggesting low-intensity treadmill exercise accelerates wound healing. The present work may provide some hint for future study of treating refractory wound.