Molecular cloning and nucleotide sequence of the DNA polymerase gene from Thermus flavus

The thermostable properties of the DNA polymerase activity from Thermus aquaticus (Taq) have contributed greatly to the yield, specificity, automation and utility of the polymerase chain reaction method for amplifying DNA. We report here the cloning and nucleotide sequence of a DNA polymerase gene f...

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Bibliographic Details
Published inNucleic acids research Vol. 20; no. 21; p. 5839
Main Authors Akhmetzjanov, A.A., Vakhitov, V.A.
Format Journal Article
LanguageEnglish
Published Oxford Oxford University Press 11.11.1992
Subjects
amino acid sequence
Base Sequence
Biological and medical sciences
Cloning, Molecular
DNA, Bacterial
DNA-directed DNA polymerase
DNA-Directed DNA Polymerase - genetics
Escherichia coli
Fundamental and applied biological sciences. Psychology
genes
Genes. Genome
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
nucleotide sequence
predictions
Thermus - enzymology
Thermus - genetics
Thermus flavus
Bacteria
DNA polymerase
Gene
Nucleotide sequence
Molecular cloning
Enzyme
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Summary:The thermostable properties of the DNA polymerase activity from Thermus aquaticus (Taq) have contributed greatly to the yield, specificity, automation and utility of the polymerase chain reaction method for amplifying DNA. We report here the cloning and nucleotide sequence of a DNA polymerase gene from another thermophilic bacterium - Thermus flavus (Tfl).
Bibliography:ark:/67375/HXZ-XZW6C4J1-J
istex:69DCED851ACE775CB1366527F9A7437AC78A8D60
ArticleID:20.21.5839
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/20.21.5839