Interaction of Graphene Oxide Nanoparticles with Human Mononuclear Cells in the Cell-IQ System

The interaction of graphene oxide nanoparticles with human peripheral blood mononuclear cells was studied using the Cell-IQ continuous monitoring system for living cells. We used graphene oxide nanoparticles of various sizes coated with linear or branched polyethylene glycol (PEG) in concentrations...

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Published inBulletin of experimental biology and medicine Vol. 175; no. 1; pp. 172 - 178
Main Authors Uzhviyuk, S. V., Khramtsov, P. V., Raev, M. B., Timganova, V. P., Bochkova, M. S., Khaziakhmatova, O. G., Malashchenko, V. V., Litvinova, L. S., Zamorina, S. A.
Format Journal Article
LanguageEnglish
Published New York Springer US 01.05.2023
Springer
Springer Nature B.V
Subjects
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cell culture
Cell growth
Drugs
Ethylenediaminetetraacetic acid
Graphene
Graphite
Graphite - pharmacology
Humans
Immune system
Immunocompetence
Intelligence
Internal Medicine
Laboratory Medicine
Leukocytes, Mononuclear
Lymphocytes
Microorganisms
Monitoring systems
Monocytes
Nanoparticles
Pathology
Peripheral blood mononuclear cells
Polyethylene glycol
Polyethylene Glycols - pharmacology
Polyols
Software
Visualization
graphene oxide nanoparticles
polyethylene glycol
cell mass increase
Cell-IQ
viability
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Summary:The interaction of graphene oxide nanoparticles with human peripheral blood mononuclear cells was studied using the Cell-IQ continuous monitoring system for living cells. We used graphene oxide nanoparticles of various sizes coated with linear or branched polyethylene glycol (PEG) in concentrations of 5 and 25 μg/ml. After 24-h incubation with graphene oxide nanoparticles, the increase in the number of peripheral blood mononuclear cells at visualization points decreased; nanoparticles coated with branched PEG more markedly suppressed cell growth in culture. In the presence of graphene oxide nanoparticles, peripheral blood mononuclear cells retained high viability in culture after daily monitoring in the Cell-IQ system. The studied nanoparticles were engulfed by monocytes and the type of PEGylation had no effect on this process. Thus, graphene oxide nanoparticles reduced the increase in peripheral blood mononuclear cell mass during dynamic observation in the Cell-IQ system without reducing their viability.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-023-05830-1