Characterization of a blaCTX-M-3, blaKPC-2 and blaTEM-1B co-producing IncN plasmid in Escherichia coli of chicken origin

• Published in: Journal of Integrative Agriculture Vol. 22; no. 1; pp. 320 - 324
• Main Authors: WANG, Wen-jing, WANG, Yi-fu, JIN, Ya-jie, SONG, Wu-qiang, LIN, Jia-meng, ZHANG, Yan, TONG, Xin-ru, TU, Jian, LI, Rui-chao, LI, Tao
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.01.2023; Guilin Medical University,Guilin 541199,P.R.China%Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,P.R.China; Hunan Provincial Key Laboratory of Tumor Microenvironment Responsive Drug Research,Institute of Pharmacy and Pharmacology,University of South China,Hengyang 421001,P.R.China; Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200241,P.R.China%Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200241,P.R.China%Hunan Provincial Key Laboratory of Tumor Microenvironment Responsive Drug Research,Institute of Pharmacy and Pharmacology,University of South China,Hengyang 421001,P.R.China; KeAi Communications Co., Ltd
• Subjects: agriculture; blaCTX-M-3; blaKPC-2; blaTEM-1B; chickens; China; drug resistance; Escherichia coli; farms; genome; genomics; hospitals; IncN; Klebsiella pneumoniae; patients; plasmid; plasmids; China; plasmid; blaCTX-M-3; IncN; blaKPC-2; blaTEM-1B; Escherichia coli
• ISSN: 2095-3119; 2352-3425
• DOI: 10.1016/j.jia.2022.08.075

An extensively drug-resistant (XDR) Escherichia coli strain 258E was isolated from an anal swab sample of a chicken farm of Anhui Province in China. Genomic analyses indicated that the strain 258E harbors an incompatibility group N (IncN) plasmid pEC258-3, which co-produces blaCTX-M-3, blaKPC-2, blaTEM-1B, qnrS1, aac(6´)-Ib-cr, dfrA14, arr-3, and aac(6´)-Ib3. Multiple genome arrangement analyses indicated that pEC258-3 is highly homologous with pCRKP-1-KPC discovered in Klebsiella pneumoniae from a patient. Furthermore, conjugation experiments proved that plasmid pEC258-3 can be transferred horizontally and may pose a significant potential threat in animals, community and hospital settings.